| Field | Specification |
|---|---|
| Target | |
| Alternative names | AF4/FMR2 family member 4|ALL1-fused gene from chromosome 5q31 protein (Protein AF-5q31)|Major CDK9 elongation factor-associated protein|AFF4|AF5Q31|MCEF |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | AF4/FMR2 family, member 4 |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Key component of the super elongation complex(SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins(ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes AFF4. It is validated for ELISA, Western Blot, Immunohistochemistry.
Validated Applications
| Applications | ELISA, Western Blot, Immunohistochemistry |
|---|---|
| WB | 1:200-1:2000 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
AF4/FMR2 family, member 4
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | AF4/FMR2 family, member 4 |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 150 kDa |
| UniProt # | Q9UHB7 |
| Gene ID | 27125 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | AF4/FMR2 family member 4|ALL1-fused gene from chromosome 5q31 protein (Protein AF-5q31)|Major CDK9 elongation factor-associated protein|AFF4|AF5Q31|MCEF |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for AFF4 research from Fine Test, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
