| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C26H29N5O7 |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
AG-7404 is an orally active, irreversible inhibitor of the picornavirus 3C protease. It blocks the processing of viral polyproteins, thereby inhibiting viral replication, and it has synergistic antiviral activity with capsid inhibitors such as V-073 or BTA798 and remains effective against V-073-resistant variants. It is used in the study of enterovirus infections such as poliovirus[1][2][3]. It is supplied as a white to off-white solid (C26H29N5O7, MW 523.54) at 98.45% purity.
Physical & Chemical Properties
| CAS Number | 343565-99-1 |
|---|---|
| Molecular Formula | C26H29N5O7 |
| Molecular Weight | 523.54 g/mol |
| Purity | 98.45% |
| Appearance | Solid |
| Color | White to off-white |
| SMILES | O=C(NCC1)[C@@H]1C[C@@H](/C=C/C(OCC)=O)NC([C@@H](N2C(C(NC(C3=NOC(C)=C3)=O)=CC=C2)=O)CC#C)=O |
| Signaling Pathway | Anti-infection |
| Solubility | In Vitro: DMSO: 25 mg/mL (47.75 mM; Requires sonication and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | -20°C, protect from light, stored under nitrogen. In solvent: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 25 mg/mL (47.75 mM) | requires sonication and warming; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); protect from light, stored under nitrogen; avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
In HeLa cells infected with poliovirus, AG-7404 (0.080-0.674 μM; 3 d) suppresses viral replication, with an EC50 of 0.080-0.674 μM[1]. Against V-073-resistant poliovirus strains, AG-7404 (0.218-0.819 μM; 3 d) stays active, giving an EC50 of 0.218-0.819 μM[1].
Cell Viability Assay[1]
| Cell Line | HeLa cells (for wild-type and vaccine-derived poliovirus strains) |
|---|---|
| Concentration | 0.080-0.674 μM |
| Incubation Time | 3 days |
| Result | Dose-dependently reduced viral cytopathic effect (CPE) as assessed by crystal violet staining and CTG assay. EC50 values were determined for each strain, demonstrating broad activity against wild-type, circulating vaccine-derived (cVDPV), and immunodeficient vaccine-derived (iVDPV) strains. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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