| Field | Specification |
|---|---|
| CAS no. | |
| Applications | |
| Molecular weight | |
| Molecular formula | C18H10N2O |
| Purity | |
| SMILES | |
| Form | Solid |
| Storage | |
| Shipping | |
| Catalog no. (Mfr.) | |
| Main SKU |
Compound Overview
AHR agonist 3 is an aryl hydrocarbon receptor (AhR) agonist that can induce cell cycle arrest or apoptosis via activation of tumor-suppressive transcriptional programs. It inhibits triple-negative breast cancer (TNBC) stem cell growth through AhR while showing minimal cytotoxicity against normal human primary cells, and it can be used for cancer research[1]. It is supplied as a light yellow to green yellow solid (C18H10N2O, MW 270.28) at 99.79% purity.
Physical & Chemical Properties
| CAS Number | 23749-58-8 |
|---|---|
| Molecular Formula | C18H10N2O |
| Molecular Weight | 270.28 g/mol |
| Purity | 99.79% |
| Appearance | Solid |
| Color | Light yellow to green yellow |
| SMILES | C1=CC=C2C(=C1)N=C3C4=CC=CC5=C4C(=CC=C5)C(=O)N23 |
| Signaling Pathway | Apoptosis; Immunology/Inflammation |
| Solubility | In Vitro: DMSO: 10 mg/mL (37.00 mM; Requires sonication and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) |
| Storage | Powder: -20°C, 3 years; 4°C, 2 years. In solvent: -80°C, 6 months; -20°C, 1 month. |
| Shipping | Room temperature in continental US; may vary elsewhere. |
Literature Cited
Sources cited in this description and in the In Vitro & In Vivo Data tab. Peer-reviewed publications that used this product are listed under References.
Safety
For Research Use Only. Not for use in diagnostic or therapeutic procedures, and not for human or veterinary use. Handle in accordance with the Safety Data Sheet and your institution's chemical hygiene plan.
In Vitro
| Solvent | Solubility | Notes |
|---|---|---|
| DMSO | 10 mg/mL (37.00 mM) | requires sonication and warming and heat to 60°C; use freshly opened DMSO (absorbed moisture lowers solubility) |
Aliquot the stock solution and store it at -80°C (up to 6 months) or -20°C (up to 1 month); avoid repeated freeze-thaw cycles.
Data provided by the manufacturer.
In Vitro
AHR agonist 3 (Analog 523) (10 nΜM; 24 hour or 2-3 weeks) triggers cell cycle arrest and reduces clonogenicity in both AhR WT and AhR KO MDA-MB-468 cells[1]. AHR agonist 3 (0-10 μΜM; 48-72 hours) promotes AhR-dependent apoptosis and growth inhibition in cancerous breast cells, but not in normal breast epithelial cells or non-tumorigenic cells, and inhibits TNBC stem cell growth via AhR[1].
Cell Proliferation Assay[1]
| Cell Line | AhR WT and AhR KO MDA-MB-468 cells |
|---|---|
| Concentration | 10 nM |
| Incubation Time | 24 hours or 2−3 weeks |
| Result | Induced an AhR-dependent S+G2/M phase arrest for Short-term (24 h) treatment. Completely exhibited the clonogenicity of AhR expressing cells while exhibiting a minimal impact on AhR deficient cells. |
Apoptosis Analysis[1]
| Cell Line | MDA-MB-468 cells, MCF10A, HEK293T and normal primary human fibroblast |
|---|---|
| Concentration | 0-10 μΜ |
| Incubation Time | 48-72 hours |
| Result | Human mammary epithelial cells (HMECs), nonmalignant mammary epithelial line Induced AhR-dependent apoptosis and growth inhibition in cancerous but not normal cells. Inhibited TNBC stem cell growth via AhR. |
Data provided by the manufacturer. Numbered citations refer to the Literature Cited list in the product description.
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