| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human Iba1 was used as the immunogen for the AIF1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
AIF1 Antibody / IBA1 is an antibody targeting AIF1. Host: Rabbit. Monoclonal. Clone: 31A74. Format: Liquid. Applications: WB, IHC, ICC, IF.
Key elements and design rationale
- Target: AIF1.
- Immunogen: A synthesized peptide derived from human Iba1 was used as the immunogen for the AIF1 antibody..
- Species reactivity: Human, Mouse, Rat.
- Conjugate: Unconjugated (no label listed).
Biological background (from provided description)
IBA1 antibody detects allograft inflammatory factor 1 (AIF1), a calcium-binding protein expressed in microglia and macrophages. IBA1 participates in actin cytoskeleton remodeling, enabling immune cells to migrate and phagocytose effectively. In the central nervous system, it serves as a widely used microglial marker, allowing researchers to monitor immune responses within brain tissue.
Common research applications
- WB: relative abundance and band pattern assessment using appropriate controls.
- IHC: tissue distribution assessment; interpret with matched controls.
- IF/ICC: subcellular localization imaging with proper background controls.
Notes for experimental interpretation
- Account for isoforms, paralogs, and post-translational modifications when interpreting signal.
- Support specificity with biological controls (e.g., KO/KD where available) and technical controls (e.g., secondary-only/isotype).
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
