| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Adenylate kinase 3 like 1; Adenylate kinase 3-like; adenylate kinase 4; Adenylate kinase isoenzyme 4; mitochondrial; AK3; AK3L1; AK3L2; AK4; ATP-AMP transphosphorylase; GTP:AMP phosphotransferase; KAD4; MGC166959; mitochondrial adenylate kinase 3; nucleoside-triphosphate adenylate kinase; |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Recombinant full length Human AK4. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
Research Use OnlyAdenylate kinases 1-5 (designated AK1-5) are a set of enzymes that regulate the phosphorylation state of intracellular adenine nucleotides, which are the principle high-energy phosphoryl-carrying molecules in living cells. AKs influence metabolic signals, which include gene expression, ion channel activity and protein kinase-mediated signaling, by catalyzing phosphoryl transfer between adenine nucleotides (AMP, ADP, ATP). Inherited mutations leading to AK deficiencies in erythrocytes have been implicated in hemolytic anemia.
Validated Applications
| Western Blot (WB) | 500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
|---|---|
| Immunohistochemistry (IHC) | 50 - 1:200 IF 1:50 - 1:200 |
| Immunofluorescence (IF/ICC) | See datasheet for recommended dilution |
Key Specifications
| Host Species | Rabbit |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 25 kDa |
| Catalog No. | BS6519 |
| UniProt | P27144 |
| Alternative Names | Adenylate kinase 3 like 1; Adenylate kinase 3-like; adenylate kinase 4; Adenylate kinase isoenzyme 4; mitochondrial; AK3; AK3L1; AK3L2; AK4; ATP-AMP transphosphorylase; GTP:AMP phosphotransferase; KAD4; MGC166959; mitochondrial adenylate kinase 3; nucleoside-triphosphate adenylate kinase; |
Immunogen / Antigen
Recombinant full length Human AK4.
Reactivity Notes
AK4 polyclonal antibody detects endogenous levels of AK4 protein.
Storage & Handling
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Safety
For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.
This antibody has been validated for: Western Blot, Immunohistochemistry, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.
WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.
Recombinant full length Human AK4.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.