AK4 polyclonal antibody

SKU:BHA11304843
Overview
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Anti-AK4 antibody (Rabbit). Validated for Western Blot, Immunohistochemistry, Immunofluorescence. Reactive against Human, Mouse, Rat targets.
Host Species Rabbit
Species Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Validated Applications Western Blot, Immunohistochemistry, Immunofluorescence
Purification Method Affinity Purification
Options selector
Catalog no. Size
BS6519-50UL 50 uL
BS6519-100UL 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL, 100 uL
  • Lead time: In Stock at Manufacturer options listed in “Availability Content”; other statuses may take longer.
  • Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at −20°C as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Mfr No BS6519
Alternative Names Adenylate kinase 3 like 1; Adenylate kinase 3-like; adenylate kinase 4; Adenylate kinase isoenzyme 4; mitochondrial; AK3; AK3L1; AK3L2; AK4; ATP-AMP transphosphorylase; GTP:AMP phosphotransferase; KAD4; MGC166959; mitochondrial adenylate kinase 3; nucleoside-triphosphate adenylate kinase;
Conjugate
  • Unconjugated
Formulation Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Host Rabbit
Immunogen Recombinant full length Human AK4.
Molecular Weight ~ 25 kDa
Product Type
  • Primary Antibody
Purity > 95% (by SDS-PAGE)
Reactivity
  • Human
  • Mouse
  • Rat
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product Overview

Research Use Only

Adenylate kinases 1-5 (designated AK1-5) are a set of enzymes that regulate the phosphorylation state of intracellular adenine nucleotides, which are the principle high-energy phosphoryl-carrying molecules in living cells. AKs influence metabolic signals, which include gene expression, ion channel activity and protein kinase-mediated signaling, by catalyzing phosphoryl transfer between adenine nucleotides (AMP, ADP, ATP). Inherited mutations leading to AK deficiencies in erythrocytes have been implicated in hemolytic anemia.

Validated Applications

Western Blot (WB) 500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Immunohistochemistry (IHC) 50 - 1:200 IF 1:50 - 1:200
Immunofluorescence (IF/ICC) See datasheet for recommended dilution

Key Specifications

Host Species Rabbit
Species Reactivity Human, Mouse, Rat
Conjugate Unconjugated
Purification Affinity Purification
Formulation Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Molecular Weight ~ 25 kDa
Catalog No. BS6519
UniProt P27144
Alternative Names Adenylate kinase 3 like 1; Adenylate kinase 3-like; adenylate kinase 4; Adenylate kinase isoenzyme 4; mitochondrial; AK3; AK3L1; AK3L2; AK4; ATP-AMP transphosphorylase; GTP:AMP phosphotransferase; KAD4; MGC166959; mitochondrial adenylate kinase 3; nucleoside-triphosphate adenylate kinase;

Immunogen / Antigen

Recombinant full length Human AK4.

Reactivity Notes

AK4 polyclonal antibody detects endogenous levels of AK4 protein.

Storage & Handling

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Safety

For Research Use Only (RUO). Not intended for diagnostic, therapeutic, or veterinary use. Handle according to standard laboratory biosafety guidelines. Avoid skin and eye contact. Dispose of waste in accordance with local regulations.

What applications has the AK4 polyclonal antibody been validated for?

This antibody has been validated for: Western Blot, Immunohistochemistry, Immunofluorescence. For each application, follow the recommended dilutions on the product datasheet and optimize empirically for your sample type.

What is the recommended dilution range?

WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200

Which species does this antibody recognize?

This antibody has been confirmed reactive against: Human, Mouse, Rat. Cross-reactivity with other species may occur due to sequence homology but is not guaranteed without independent validation.

What is the immunogen / antigen used to generate this antibody?

Recombinant full length Human AK4.

How should this antibody be stored and handled?

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Avoid repeated freeze-thaw cycles. Centrifuge vials briefly before opening. Dilute immediately before use.

Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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