AKAP 95 Polyclonal Antibody

SKU:BHA12100232
Overview
Click light‑blue chips for details
AKAP8-specific polyclonal antibody raised in Rabbit. Validated for Western Blot, Immunohistochemistry, Immunofluorescence in Human, Mouse, Rat samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen The antiserum was produced against synthesized peptide derived from human AKAP8. AA range:331-380
Molecular Weight ~76.1 kDa
UniProt O43823
Gene ID Human: 10270; Mouse: 56399; Rat: 116633
Applications 4 validated
Available Options
Size
Options selector
Catalog no. Size
BT-AP00327-20UL 20 uL
BT-AP00327-50UL 50 uL
BT-AP00327-100UL 100 uL
Field Specification
Alternative Names AKAP8; AKAP95; A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95
Clonality
  • Polyclonal
Concentration 1 mg/ml
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Gene ID Human: 10270; Mouse: 56399; Rat: 116633
Host Rabbit
Immunogen The antiserum was produced against synthesized peptide derived from human AKAP8. AA range:331-380
Isotype
  • IgG
Molecular Weight ~76.1 kDa
Product Type
  • Primary Antibody
Reactivity
  • Human
  • Mouse
  • Rat
Storage -20°C for 1 year
Target AKAP8

Product Overview

Primary Antibody

This antibody targets AKAP8 and detects endogenous levels of AKAP8 protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC) Refer to datasheet for recommended working dilution
Immunofluorescence (IF) Refer to datasheet for recommended working dilution
ELISA Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target AKAP8
Host Rabbit
Clonality Polyclonal
Isotype IgG
Reactivity Human, Mouse, Rat
Purification Affinity Purification
Molecular Weight ~76.1 kDa
Concentration 1 mg/ml
Immunogen The antiserum was produced against synthesized peptide derived from human AKAP8. AA range:331-380
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -20°C for 1 year
Gene ID Human: 10270; Mouse: 56399; Rat: 116633
UniProt O43823 (Human: O43823; Mouse: Q9DBR0; Rat: Q63014)

Scientific Background

AKAP8 encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. ThAKAP8 encodes a nuclear A-kinase anchor protein (A-kinase anchoring protein 8) that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of AKAP8 is located on the short arm of chromosome 9.

Alternative Names

AKAP8; AKAP95; A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetAKAP8
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ReactivityHuman, Mouse, Rat
PurificationAffinity Purification
Molecular Weight~76.1 kDa
Concentration1 mg/ml
ImmunogenThe antiserum was produced against synthesized peptide derived from human AKAP8. AA range:331-380
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20°C for 1 year
Gene IDHuman: 10270; Mouse: 56399; Rat: 116633
UniProtO43823 (Human: O43823; Mouse: Q9DBR0; Rat: Q63014)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC)Refer to datasheet for recommended working dilution
Immunofluorescence (IF)Refer to datasheet for recommended working dilution
ELISARefer to datasheet for recommended working dilution

AKAP8 encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. ThAKAP8 encodes a nuclear A-kinase anchor protein (A-kinase anchoring protein 8) that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of AKAP8 is located on the short arm of chromosome 9.

Alternative Names

AKAP8; AKAP95; A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95

What species does this antibody react with?
This antibody has been validated for reactivity against Human, Mouse, Rat. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA. Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This polyclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of AKAP8?
The predicted molecular weight of AKAP8 is ~76.1 kDa. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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