| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB, RAC |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthetic phosphopeptide derived from human AKT around the phosphorylation site of Threonine 308. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
AKT (phospho-T308) polyclonal antibody detects endogenous levels of AKT protein only when phosphorylated at Thr308.
Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | 1:2000~1:5000 |
| Immunoprecipitation (IP) | 1:50~1:200 |
| Immunofluorescence (IF) | 1:50~1:200 |
Product Specifications
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Isotype | IgG |
| Species Reactivity | Human, Rat, Mouse |
| Conjugate | Unconjugated |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
| Molecular Weight | ~ 60 kDa |
| Modification | Phosphorylated |
| UniProt / Swiss-Prot | P31749/P31751/Q9Y243 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Signaling Pathway(s) | Phosphoinosotode Signaling; PI3K AKT signaling; Protein Kinase C Signaling; Immune Cell Signaling; Warburg Effect; Translational Contral elF4 and p70 S6 Kinase; Regulation of Microtubule Dynamics; T cell receptor signaling; Inhibition of Apoptosis; Insulin &Glucose Signaling; Cell Cycle Control G1 S Checkpoint; Adherens Junction signaling; Mitochondrial Control of Apoptosis; MTOR signaling; ErbB HER Signaling |
Immunogen / Antigen
Synthetic phosphopeptide derived from human AKT around the phosphorylation site of Threonine 308.
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
Related Products
Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for WB, IP, IF. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Rat, Mouse. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Rabbit. For indirect detection (WB, IHC, IF), select an anti-Rabbit secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Synthetic phosphopeptide derived from human AKT around the phosphorylation site of Threonine 308.
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Rat, Mouse samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
