| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | RAC-alpha serine/threonine-protein kinase; Protein kinase B; PKB; Protein kinase B alpha; PKB alpha; Proto-oncogene c-Akt; RAC-PK-alpha; AKT1; PKB; RAC; PKBα; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; PKB beta; RAC-PK-beta; AKT2; PKBβ; RAC-gamma serine/threonine-protein kinase; Protein kinase Akt-3; Protein kinase B gamma; PKB gamma; RAC-PK-gamma; STK-2; AKT3; PKBG; PKBγ |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 308. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
p-AKT (T308) polyclonal antibody detects endogenous levels of AKT1 only when phosphorylated at Thr308. This antibody also recognizes AKT2 and AKT3 when phosphorylated at the corresponding residues.
AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Western Blot (WB) | 1:500~1:1000 |
| Immunohistochemistry (IHC) | 1:50~1:100 |
Product Specifications
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Isotype | IgG |
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 55 kDa |
| Modification | Phosphorylated |
| UniProt / Swiss-Prot | P31749/P31751/Q9Y243 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Signaling Pathway(s) | Phosphoinosotode Signaling; PI3K AKT signaling; Protein Kinase C Signaling; Immune Cell Signaling; Warburg Effect; Translational Contral elF4 and p70 S6 Kinase; Regulation of Microtubule Dynamics; T cell receptor signaling; Inhibition of Apoptosis; Insulin &Glucose Signaling; Cell Cycle Control G1 S Checkpoint; Adherens Junction signaling; Mitochondrial Control of Apoptosis; MTOR signaling; ErbB HER Signaling |
Immunogen / Antigen
Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 308.
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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Explore complementary antibodies, ELISA kits, and detection reagents at BioHippo.
This antibody has been validated for WB, IHC. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Mouse, Rat. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Rabbit. For indirect detection (WB, IHC, IF), select an anti-Rabbit secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Threonine 308.
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Mouse, Rat samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
