| Field | Specification |
|---|---|
| Target | |
| Alternative names | Akt3; AKT3 kinase; AKT3_HUMAN; DKFZp434N0250; MPPH; PKB gamma; PKBG; PRKBG; Protein kinase Akt-3; Protein Kinase AKT3; Protein kinase B gamma; RAC gamma; RAC gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; RACPK Gamma; Serine threonine protein kinase Akt 3; Serine threonine protein kinase Akt3; STK 2; STK-2; STK2; V akt murine thymoma viral oncogene homolog 3 (protein kinase B; gamma); V akt murine thymoma viral oncogene homolog 3; V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human RAC-gamma serine/threonine-protein kinase protein (1-164AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-AKT3 antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect AKT3 in Human for signal transduction research.
Key elements and design rationale
- Immunogen: Recombinant Human RAC-gamma serine/threonine-protein kinase protein (1-164AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- HRP: Direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
Biological background
AKT3 (also: Akt3, AKT3 kinase, AKT3_HUMAN, DKFZp434N0250, MPPH, PKB gamma, PKBG, PRKBG, Protein kinase Akt-3, Protein Kinase AKT3, Protein kinase B gamma, RAC gamma, RAC gamma serine/threonine protein kinase, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, RACPK Gamma, Serine threonine protein kinase Akt 3, Serine threonine protein kinase Akt3, STK 2, STK-2, STK2, V akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma), V akt murine thymoma viral oncogene homolog 3, V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current AKT3 annotation relevant to signal transduction.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
AKT3 is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects AKT3 in ELISA applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
HRP: direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing AKT3; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
