Alab cell

SKU:BHC11101019
Bulk Pricing
Overview
Click light‑blue chips for details

Alab cell is a cell line (Male). It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent,suspension. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Human
Disease model Adenocarcinoma
Growth Properties Adherent,suspension
Tissue Breast
Options selector
Catalog no. Size
300280 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 300280
Species Human
The ALAB cell line is a human mammary adenocarcinoma cell line derived from a mammary tumor. It has been adapted to grow in vitro, particularly on collagen substrates, which facilitates the study of tumor cell behavior in mammary carcinomas. ALAB cells are notably used in research focused on calcium-binding and collagen-binding proteins (CaBP and CBP, respectively). In these cells, the calcium-binding proteins were isolated and analyzed, revealing a significant 38 kDa protein, which is closely associated with annexins, a family of proteins involved in cellular processes such as membrane trafficking and signal transduction. One of the key proteins identified in ALAB cells is annexin II, a calcium-dependent protein that binds to collagen and plays a role in various cellular functions, including exocytosis and cytoskeletal organization. Immunofluorescence studies of ALAB cells reveal a perinuclear granular pattern of annexin II expression, indicating its involvement in protein secretion and cellular differentiation. The 38 kDa annexin II protein detected in these cells is also associated with collagen-binding properties, which can be crucial for tumor progression and metastasis, making ALAB a valuable model for studying mammary tumor biology and protein interactions.

SKU:BHC11101019

  • cultureMedium: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
  • supplements: Supplement the medium with 5% FBS
  • dissociationReagent: Accutase
  • subculturing: Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium.
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Matches this product
10% OFF
10% OFF CELL LINES-Limited-Time Offer
Ends Sep 30 CELL10
15%OFF
15% Off Cancer Antibodies
Ends Sep 30 ONCO15
$50 OFF
$50 Off All ELISA Kits
Limited time ELISA50
FREE SAMPLE
Free Sample – CellTrypase Recombinant Trypsin-Like Enzyme
Limited time offer – availa... FREESAMPLE
FREE SAMPLE
Free Sample – MycoFold™ Growth Factors
Limited time offer – availa... Request Free Sample

Get a Quote

Please use this form for bulk quantity requests or customized products.

Contact Information

Product Information

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today

Try Celltrypse Free – Request Your Sample Today

Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

Try Celltrypse Free – Request Your Sample Today