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Aldosterone is a mineralocorticoid first isolated from beef adrenal glands. It controls the sodium-potassium balance through the unidirectional salt reabsorption in a variety of tissues and glands. Peripheral aldosterone levels are dependent on age and body position with an expected aldosterone level of less than 300 pg/mL being found in a typical upright adult.
Aldosterone measurement is useful in the investigation of primary aldosteronism (i.e. adrenal, adenoma or carcinoma, and adrenal cortical hyperplasia) and secondary aldosteronism (renovascular disease, salt depletion, potassium loading, cardiac failure with ascites, pregnancy, and Bartter syndrome).
Assay Principle
Tribioscience’s Aldosterone ELISA kit is a competitive ELISA assay for the quantitative measurement of aldosterone in extracted serum, extracted plasma, urine, extracted dried fecal samples, and tissue culture media samples.
Research use only. Additional Assay Data Specificity of Assay
Aldosterone Competitive ELISA Kit is a Competitive ELISA for the quantitative determination of Aldosterone. In this competitive ELISA, sample analyte competes with a labeled competitor for a limited number of antibody binding sites, so signal is inversely proportional to analyte concentration.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Aldosterone |
|---|---|
| Assay Type | Competitive |
| Test Principle | Competitive ELISA |
| Storage | Store the unopened kit at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this competitive ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
