Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L)

SKU:BHA10575518
Overview
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IgG-specific polyclonal antibody raised in Donkey. Alexa Fluor 647-conjugated. Validated for Flow Cytometry in Mouse samples. Affinity-purified.
Conjugate Alexa Fluor 647
Immunogen IgG purified from mouse serum
Applications 1 validated
Dilution FC:1:100-1:800
Available Options
Size
Options selector
Catalog no. Size
CSB-PA439483-100UL 100 uL
CSB-PA439483-500UL 500 uL
Field Specification
Antigen
  • IgG purified from mouse serum
Clonality
  • Polyclonal
Conjugate
  • Alexa Fluor 647
Form Liquid
Host Donkey
Immunogen IgG purified from mouse serum
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Mouse
Source This product is a polyclonal antibody purified from donkey antiserum.
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target IgG

Product Overview

Primary Antibody

This antibody targets IgG and was raised against a recombinant protein immunogen.

Validated Applications

Application Recommended Dilution
Flow Cytometry (FC) FC:1:100-1:800
✔ Validated by CUSABIO Technology

Antibody Specifications

Target IgG
Host Donkey
Clonality Polyclonal
Isotype IgG
Conjugate Alexa Fluor 647
Reactivity Mouse
Purification Affinity Purification
Antigen / Immunogen IgG purified from mouse serum
Production This product is a polyclonal antibody purified from donkey antiserum.
Form Liquid
Storage Buffer 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Safety & Handling

⚠ Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle with standard laboratory precautions. Dispose per local regulations.
TargetIgG
HostDonkey
ClonalityPolyclonal
IsotypeIgG
ConjugateAlexa Fluor 647
ReactivityMouse
PurificationAffinity Purification
Antigen/ImmunogenIgG purified from mouse serum
ProductionThis product is a polyclonal antibody purified from donkey antiserum.
FormLiquid
Storage Buffer0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
StorageUpon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Dilutions are starting recommendations. Titration is advised for each system.

ApplicationRecommended Dilution
Flow Cytometry (FC)FC:1:100-1:800

Immunogen: IgG purified from mouse serum

Antigen Species: Mouse

What species does this IgG antibody react with?
This antibody has been validated for reactivity against Mouse. Cross-reactivity is based on the immunogen sequence and may vary by sample type.
What applications has this antibody been validated for?
Validated applications include: Flow Cytometry (FC). Each application was confirmed with appropriate controls. Contact technical support for applications not listed.
What is the recommended dilution?
FC:1:100-1:800. Optimal concentrations depend on sample type, detection system, and antigen abundance. Titration with positive and negative controls is recommended.
How was this antibody produced?
This polyclonal IgG antibody was raised in Donkey and purified by affinity chromatography using Protein G. It provides high specificity and minimal cross-reactivity in standard immunoassay applications.
What are the storage requirements?
Store at −20°C for long-term. Avoid repeated freeze-thaw cycles. Short-term working aliquots may be stored at 4°C. This product is supplied in a stabilized buffer containing BSA and sodium azide.
Is this antibody directly conjugated and does it require secondary detection?
Yes, this antibody is Alexa Fluor 647-conjugated and can be used directly without a secondary antibody. It is ready to use in assays requiring Alexa Fluor 647 detection, such as flow cytometry or direct ELISA applications. Ensure your detection instrument is calibrated for Alexa Fluor 647 signal.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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