| Field | Specification |
|---|---|
| Mfr No | |
| Form | 5X Mastermix |
| Product Type | |
| Storage |
The All-In-One 5X RT MasterMix with gDNA Removal offers a convenient, ready-to-use solution for first-strand cDNA synthesis with genomic DNA removal, all in a single tube. This powerful formulation addresses the common challenge of genomic DNA contamination, ensuring accurate RNA detection without compromising reverse transcription or cDNA synthesis.
The optimized MasterMix includes abm’s proprietary OneScript® Hot Reverse Transcriptase, RNaseOFF Ribonuclease Inhibitor, temperature-sensitive DNase, dNTPs, and a carefully balanced mix of Oligo(dT)s and Random Primers. The OneScript® Hot Reverse Transcriptase features genetic modifications that abolish RNase H activity to achieve thermal stability, allowing the RNA template to remain intact during reverse transcription for enhanced cDNA yield and integrity. By eliminating the need for multiple separate reagents, this all-in-one mix offers a straightforward, reliable, and reproducible workflow that consistently delivers high-quality cDNA, even from challenging or low-quality RNA samples—ideal for a wide range of downstream molecular biology applications.
Product Features:
- Easy one-step setup reduces pipetting and sample handling
- Removes contaminating gDNA with temperature-sensitive DNase
- High cDNA yields from difficult samples with OneScript® Hot Reverse Transcriptase (Cat. No. G593)
- Inhibits ribonuclease contaminants with RNaseOFF Ribonuclease Inhibitor (Cat. No. G138)
- Also includes dNTPs, Oligo (dT)s, and random primers
- Included in abm's PCR Buffet Program
| Product Component | Quantity |
|---|---|
| All-In-One 5X RT MasterMix | 100 rxn (400 µl) |
| Nuclease-Free H2O | 1.0 ml |
 |
ISO 13485:2016 MDSAP Certified |
| Component | Quantity |
|---|---|
| All-In-One 5X RT MasterMix | 100 rxn (400 µl) |
| Nuclease-Free H2O | 1.0 ml |
| Specification | Value |
|---|---|
| Enzyme Type | RT Mastermix |
| Format | 5X Mastermix |
| Storage Conditions | Store at -20°C. |
The kit includes everything you need for cDNA synthesis in one tube: All-In-One 5X RT MasterMix (100 reactions, 400 µl), which contains reverse transcriptase, RNaseOFF RNase inhibitor, DNase, dNTPs, and primers. Additionally, a tube of Nuclease-Free H₂O (1.0 ml) is provided to complete your reaction setup.
Store the All-In-One 5X RT MasterMix with gDNA Removal at -20°C. This product is unstable if stored improperly. Reduce freeze-thaw cycling and handle with care.
Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
To improve genomic DNA removal, increase the 37°C incubation time from 15 minutes to 30 minutes.
Yes, for longer RNA transcripts, extend the 60°C incubation time to up to 30 minutes.
If genomic DNA removal is not required, use the OneScript® Hot 5X RT MasterMix (Cat. No. G590) for a faster and more efficient cDNA synthesis process.
The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
Store the synthesized first-strand cDNA at -20°C for long-term use.
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