| Field | Specification |
|---|---|
| Target | |
| Alternative names | E3 ubiquitin-protein ligase AMFR|Autocrine motility factor receptor (AMF receptor)|RING finger protein 45|gp78|AMFR|RNF45 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | autocrine motility factor receptor |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation(ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes AMFR. It is validated for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation.
Validated Applications
| Applications | ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation |
|---|---|
| WB | 1:500-1:5000 |
| IF | 1:10-1:100 |
| IHC | 1:20-1:200 |
| IP | 1:200-1:2000 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
autocrine motility factor receptor
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | autocrine motility factor receptor |
| Purification | Immunogen Affinity Purified |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 73 kDa |
| UniProt # | Q9UKV5 |
| Gene ID | 267 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | E3 ubiquitin-protein ligase AMFR|Autocrine motility factor receptor (AMF receptor)|RING finger protein 45|gp78|AMFR|RNF45 |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for AMFR research from Fine Test, or contact us for alternative formats.
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