| Field | Specification |
|---|---|
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Product Overview
The kit extracts high purity total DNA from blood clot samples as well as from animal tissues, cells, blood and bacteria. It is suitable for 100 µL–1 mL blood clot samples and purifies DNA fragments up to 50 kb in length. The purification process does not require toxic solvents such as phenol or chloroform, and ethanol precipitation is not required. Purified DNA can be used directly in downstream experiments such as digestion, PCR, Real-Time PCR, library construction and Southern hybridization.
The blood clot is lysed with 1–2.5 volumes of Solution RCL, then 200 µL of Solution GAS1, 20 µL of Proteinase K and 300 µL of Solution GA2 are added and the sample is incubated at 56°C for 10 min. After adding 300 µL of absolute ethanol the lysate is loaded onto Adsorption column G, washed with 500 µL of Buffer RP and twice with 600 µL of Wash Buffer, dried by centrifugation, and eluted with 50–200 µL of Solution GE after 2–5 min at room temperature. Purified DNA has an OD260/OD280 of 1.7–1.9.
Specifications
| Format | Silica-based spin column (Adsorption column G) |
|---|---|
| Sample types | Blood clot; also animal tissue, cells, blood and bacteria |
| Sample input | 100 µL–1 mL blood clot |
| Maximum fragment size | 50 kb |
| Processing time | Within one hour |
| Chemistry | Phenol/chloroform-free; no ethanol precipitation |
| Purity | OD260/OD280 should be 1.7–1.9 |
| Elution volume | 50–200 µL of Solution GE |
Kit Components
| Component | 50 T | 200 T |
|---|---|---|
| Proteinase K | 1 mL | 4 mL |
| Solution GAS1 | 25 mL | 100 mL |
| Solution GA2 | 25 mL | 100 mL |
| Wash Buffer | 60 mL | 240 mL |
| Buffer RP | 30 mL | 120 mL |
| Solution RCL | 15 mL | 60 mL |
| Solution GE | 10 mL | 40 mL |
| Adsorption column G | 50 sets | 200 sets |
Applications
- Genomic DNA extraction from blood clot samples
- Enzyme digestion, PCR and Real-Time PCR
- Library construction
- Southern hybridization
Key Features
- Simple and fast: High purity genomic DNA can be obtained within one hour.
- High purity: The obtained DNA is of high purity and can be directly used in molecular biology experiments such as PCR, enzyme digestion, and hybridization.
Storage & Handling
Proteinase K is stored at -20°C for 1 year. Solutions GAS1, GA2, RCL and GE, the Wash Buffer, Buffer RP and Adsorption column G are stored at room temperature for 1 year.
Usage Notes
- Avoid repeated freeze–thaw cycles.
- Dissolve precipitated Solution GAS1 or Solution GA2 at 56°C before use.
- Add RNase A separately if RNA removal is required; it is not supplied in the kit.
- Add ethanol to the buffers before use.
- Remove residual ethanol before elution to avoid inhibition of downstream enzymes.
Safety & Regulatory
For research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.
Customization & Add-ons: Need this molecular biology reagent tailored to your workflow? We can help with bulk or custom pack sizes, alternative formats (spin column, magnetic bead or reagent-only kits; premixed or stand-alone reference dye), matched extraction, cDNA synthesis and qPCR workflows, and sourcing of related enzymes, ladders and consumables not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
