| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 5'-AMP-activated protein kinase subunit gamma-1; AMPK gamma1; AMPK subunit gamma-1; AMPKg; PRKAG1; AMPKγ1; AMPK-γ1; 5'-AMP-activated protein kinase subunit gamma-2; AMPK gamma2; AMPK subunit gamma-2; H91620p; PRKAG2; AMPKγ2; AMPK-γ2; 5'-AMP-activated protein kinase subunit gamma-3; AMPK gamma3; AMPK subunit gamma-3; PRKAG3; AMPKG3; AMPKγ3; AMPK-γ3; |
| Conjugate | |
| Formulation | |
| Host | |
| Immunogen | Synthetic peptide, corresponding to amino acids 50-100 of Human AMPKγ1. |
| Molecular Weight | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage |
Product Overview
AMPKγ1/2/3 (K78) polyclonal antibody detects endogenous levels of total AMPKγ protein.
The 5-AMP activated protein kinase (AMPK), a member of the SNF1 (sucrose non fermentor) kinase family, is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase.
Validated Applications
| Application | Recommended Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1:50~1:200 |
| Immunofluorescence (IF) | 1:50~1:200 |
Product Specifications
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Isotype | IgG |
| Species Reactivity | Human, Mouse, Rat |
| Conjugate | Unconjugated |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
| Molecular Weight | ~ 38,54,63 kDa |
| Modification | Unmodified |
| UniProt / Swiss-Prot | P54619/Q9UGJ0/Q9UGI9 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Signaling Pathway(s) | Dopamine Signaling in Parkinson's Disease Plathway |
Immunogen / Antigen
Synthetic peptide, corresponding to amino acids 50-100 of Human AMPKγ1.
Safety & Handling
For Research Use Only (RUO). Not for diagnostic or therapeutic use. Handle as a potential biohazard in accordance with good laboratory practices.
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This antibody has been validated for IHC, IF. Always refer to the product datasheet for recommended dilution ranges and appropriate positive control samples for each application type.
Validated reactivity has been confirmed for Human, Mouse, Rat. Cross-reactivity with additional species may exist based on sequence homology but has not been confirmed by the manufacturer. For unlisted species, we recommend running a preliminary validation experiment.
This antibody was raised in Rabbit. For indirect detection (WB, IHC, IF), select an anti-Rabbit secondary antibody that matches the isotype. For direct detection, check the variant selector for conjugated formats. Confirm secondary antibody compatibility before ordering.
Synthetic peptide, corresponding to amino acids 50-100 of Human AMPKγ1.
Please refer to the storage conditions on the product label and datasheet. As a general best practice: upon receipt, aliquot into single-use volumes to minimize freeze-thaw cycling, which can progressively reduce antibody performance and lead to aggregation.
Validated reactivity covers Human, Mouse, Rat samples. Performance can vary across different cell lines and tissue types depending on target antigen expression levels and epitope accessibility. We recommend verifying target expression in your specific model system using a known positive control before proceeding to experimental samples.
BioHippo offers customization and value-added services for qualified research programs:
- Bulk Pricing: Volume discounts available for orders of 5 or more units. Request a quote through the Contact Us page.
- Custom Conjugation: Select antibody products are available for custom conjugation to fluorophores, biotin, HRP, or other detection labels upon request. Minimum quantities apply.
- Custom Formats: Lyophilized or concentrated formats may be available for select products. Inquire before ordering.
- Technical Support: Our scientific team is available to assist with application optimization, protocol troubleshooting, and experimental design.
For customization inquiries, please use the Contact Us page or reach out to our support team directly.
