| Field | Specification |
|---|---|
| Mfr No | |
| Activity | |
| Cas No. | |
| Concentration | |
| Form | Lyophilized Powder |
| Product Type | |
| Purity | |
| Reconstitution | |
| Shipping | |
| Solubility | DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min). |
| Source | Natural |
| Storage |
Product details
- Chemical name: (2R,3S,4S)-2-[(4-Methoxyphenyl)methyl]-3,4-pyrrolidinediol 3-acetate.
- Also known as: Flagecidin
- CAS number: 22862-76-6
- Molecular formula: C14H19NO4.
- Purity: >98%
- Concentration: 0.1-10 µM.
- Form: Lyophilized Powder
- Solubility: DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
- Reconstitution: DMSO or ethanol. Centrifuge all product preparations before use (10000 x g 5 min).
- Shipping: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C.
- Source: Natural
- Activity: Anisomycin is an antibiotic which inhibits protein synthesis1. It also activates stress-activated protein kinases (SAPK) independent of its ability to block translation.
- Alternative names: Flagecidin
Scientific background
Anisomycin is a bacterial antibiotic isolated from Streptomyces griseolus, which inhibits protein synthesis by binding to 60S ribosomal subunits and blocking peptide bond formation, thereby preventing elongation and causing polysome stabilization. This action of Anisomycin monitored the cells for chromosomal DNA degradation and apoptosis.1-5Anisomycin has been widely used as an extremely potent activator of kinase cascades in mammalian cells, especially the stress-activated protein kinase (SAPK2/p38MAPK), kinase subtypes and p46/54JNK.6-13Synergizes with growth factors and phorbol esters, anisomycin superinduce c-fos and c-jun by a number mechanisms, one of which is its ability to act as a potent signalling agonist, producing strong, prolonged activation of the same nuclear responses as epidermal growth factor or tetradecanoyl phorbol acetate.14Anisomycin stimulated serine phosphorylation of IRS-1 and IRS-2, reduced their ability to interact with the insulin receptor and by that blocked the insulin-induced tyrosine phosphorylation of IRS proteins.15
Lead time: 1-2 Business Days
Country of origin: Israel/IL
Applications key
Application key: FC- Flow cytometry, IFC- Indirect flow cytometry, IHC- Immunohistochemistry,LCI- Live cell imaging, Calcium imaging assay,Cell survival assay, Electrophysiology, Neurite outgrowth assay.
Bioassay tested: Yes
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- Add-on labels & handles: D/¹³C/¹⁵N isotopes (LC-MS/internal standards), azide/alkyne or other functional handles for conjugation
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