| Field | Specification |
|---|---|
| Target | |
| Alternative names | Arachidonate 12-lipoxygenase, 12S-type;12S-LOX;12S-lipoxygenase;1.13.11.31;Lipoxin synthase 12-LO;3.3.2.-;Platelet-type lipoxygenase 12;ALOX12;12LO, LOG12; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human 12 Lipoxygenase, different from the related mouse sequence by six amino acids, and from the related rat sequence by seven amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes polyunsaturated fatty acid lipoxygenase ALOX12 in mouse and rat samples and is validated for Western blot. The predicted molecular weight is 75.7 kDa, and the supplier reports an observed band at about 80 kDa.
Platelet-type 12-lipoxygenase (ALOX12) is a non-heme iron enzyme that oxidizes arachidonic acid to 12S-HPETE, which is reduced to 12-HETE. These lipid mediators influence platelet activation, inflammation and vascular function, and ALOX12 is also studied in skin barrier biology and cancer.
| Target | Polyunsaturated fatty acid lipoxygenase ALOX12 (gene ALOX12; UniProt P18054, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 75.7 kDa |
| Observed MW | 80 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Mouse, Rat) |
|---|
Samples with a confirmed band (WB): rat spleen tissue, mouse spleen tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the N-terminal region of human 12 Lipoxygenase. It differs from the mouse sequence at six residues and from the rat sequence at seven residues.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places polyunsaturated fatty acid lipoxygenase ALOX12 in the cytoplasm and membrane. Tissue expression noted by UniProt (human): Expressed in vascular smooth muscle cells. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Bryan Harris et al. (2025) Ribosomal protein control of hematopoietic stem cell transformation through regulation of metabolism. Cell Reports. 10.1016/j.celrep.2025.116688
