| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Arachidonate 12-lipoxygenase, 12S-type;12S-LOX;12S-lipoxygenase;1.13.11.31;Lipoxin synthase 12-LO;3.3.2.-;Platelet-type lipoxygenase 12;ALOX12;12LO, LOG12; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human 12 Lipoxygenase, different from the related mouse sequence by six amino acids, and from the related rat sequence by seven amino acids. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ALOX12 (Arachidonate 12-lipoxygenase, 12S-type) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-12 Lipoxygenase/ALOX12 Antibody Picoband® catalog # A02275-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: A synthetic peptide corresponding to a sequence at the N-terminus of human 12 Lipoxygenase, different from the related mouse sequence by six amino acids, and from the related rat sequence by seven amino acids.
- Molecular weight context: reported MW: 80 kDa; calculated MW: 75694 MW
- Reactivity: Mouse,Rat
- Applications: WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Arachidonate 12-lipoxygenase, 12S-type; Arachidonate 12-lipoxygenase, 12S-type. ALOX12, Arachidonate 12-lipoxygenase, is an enzyme that in humans is encoded by the ALOX12 gene. By fluorescence in situ hybridization, the ALOX12 gene is located in band 17p13.1. The gene consists of 14 exons with 13 introns and spans approximately 15 kb of DNA Arachidonate 12-lipoxygenase introduces a molecular oxygen into the C-12 position of arachidonic acid to produce 12 (S)-hydroperoxy-5,8,10,14-eicosatetraenoic acid. The major pathway of arachidonic acid metabolism in human platelets proceeds via a 12-lipoxygenase enzyme. Expression of the LOG12 gene was detected in human erythroleukemia cells, platelets, and human umbilical vein endothelial cells by reverse transcription-PCR analysis. Functional note: Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)- hydroperoxyeicosatetraenoic acid/ (12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation. . Reported localization: Cytoplasm, cytosol. Membrane. Membrane association is stimulated by EGF. Expression/tissue context: Expressed in vascular smooth muscle cells. .
Research relevance and current trends
- Cardiovascular: Researchers commonly examine how ALOX12 (Arachidonate 12-lipoxygenase, 12S-type) relates to this theme using model systems and orthogonal readouts.
- Cell Cycle: Researchers commonly examine how ALOX12 (Arachidonate 12-lipoxygenase, 12S-type) relates to this theme using model systems and orthogonal readouts.
- Cell Differentiation: Researchers commonly examine how ALOX12 (Arachidonate 12-lipoxygenase, 12S-type) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ALOX12 (Arachidonate 12-lipoxygenase, 12S-type) levels across conditions; band patterns may reflect isoforms and processing.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins.
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
