| Field | Specification |
|---|---|
| Target | |
| Alternative names | 14-3-3 protein gamma;Protein kinase C inhibitor protein 1;KCIP-1;14-3-3 protein gamma, N-terminally processed;YWHAG; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human 14-3-3 gamma |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone HAH-25 is a rabbit monoclonal antibody that recognizes 14-3-3 protein gamma (gene YWHAG) in human, mouse and rat samples and is validated for Western blot and flow cytometry. The predicted molecular weight is 28.3 kDa, and the supplier reports an observed band at about 28 kDa.
14-3-3 γ (YWHAG) is a member of the 14-3-3 family of adapter proteins that bind phosphoserine/phosphothreonine motifs on many signaling proteins. It is highly expressed in brain and muscle and helps regulate kinase signaling, cell-cycle control and neuronal development, and YWHAG variants have been linked to epilepsy.
| Target | 14-3-3 protein gamma (gene YWHAG; UniProt P61981, human) |
|---|---|
| Clone | HAH-25 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 28.3 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): HeLa cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human 14–3–3 gamma.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 14-3-3 protein gamma in the cytoplasm and mitochondrion matrix. Tissue expression noted by UniProt (human): Highly expressed in brain, skeletal muscle, and heart. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
