Anti-2B4/Cd244 Antibody Picoband®

Overview

Anti-2B4/Cd244 Antibody Picoband® is an antibody for Cd244 detection raised in Rabbit (Polyclonal, Rabbit IgG), with reported reactivity: Rat. Commonly used in WB, IHC, Flow Cytometry, ELISA workflows.

Key elements and design rationale

• Target: Cd244 (CD244 molecule); UniProt: E9PT21
• Antibody format: Rabbit, Polyclonal, Rabbit IgG
• Molecular weight: 50 kDa, calculated 87164 MW
• Applications: WB, IHC, Flow Cytometry, ELISA

Vendor description (summary): Boster Bio Anti-2B4/Cd244 Antibody Picoband® catalog # A02527-2.

Biological background

Biological context: Modulate other receptor-ligand interactions to enhance leukocyte activation.

Expression and localization notes: cellular localization: integral component of membrane, tissue context: Expressed in natural killer (NK) cells, T cells and dendritic cells..

Common research applications

• Western blotting (WB): Compare Cd244 levels across samples and conditions using appropriate loading and biological controls.
• Immunohistochemistry (IHC): Evaluate spatial distribution of Cd244 in tissue sections, considering fixation and antigen retrieval effects.
• Flow cytometry: Quantify Cd244-positive populations in single-cell suspensions with appropriate gating and controls.
• ELISA: Use antibody-based detection formats to assess antigen presence or binding in plate-based assays.

Notes for experimental interpretation

• Account for isoforms, post-translational modifications, and sample-specific processing that can shift apparent molecular weight or epitope accessibility.
• Use positive/negative biological controls where possible (e.g., known-expressing cells/tissues, knockdown/knockout models) and include appropriate secondary-only/isotype controls for imaging workflows.

Additional product notes (from provided fields)

• Specificity: No cross reactivity with other proteins.
• Background: CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Tangye et al. (1999) mapped the 2B4 gene to 1q22. Suzuki et al. (2008) identified a functional single-nucleotide polymorphism (SNP) in the CD244 gene that contributes to rheumatoid arthritis susceptibility. Functional analysis by Boles et al. (1999) demonstrated that engagement of 2B4 with specific antibody activates NK cytolytic activity. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, Brown et al. (1998) demonstrated that CD48 binds to 2B4. Watzl et al. (2000) showed that antibody-mediated cross-linking of 2B4 leads to its rapid tyrosine phosphorylation, which is necessary for 2B4-mediated killer cell activity.
• Cross reactivity: No cross-reactivity with other proteins.
• Cellular localization: integral component of membrane
• Tissue details: Expressed in natural killer (NK) cells, T cells and dendritic cells.
• Research category: Cancer,Cancer Metabolism,Cardiovascular,Growth Factors/Hormones,Metabolism,Metabolism Processes,Pathways and Processes,Response To Hypoxia,Signal Transduction,Vasculature

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.