Anti-2B4/Cd244 Antibody Picoband®

SKU:BHA21002402
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Suppliers
Boster Bio
Boster Bio
Details Products
Overview
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Anti-Cd244 antibody (Rabbit polyclonal, Rabbit IgG). Validated for Western blot and ELISA. Reactivity: Rat. Recognizes CD244 molecule. Supplied in lyophilized form, ready for use in standard immunodetection workflows.
Target Cd244
Host Rabbit
Reactivity Rat
Isotype Rabbit IgG
Application(s) WB, IHC, Flow Cytometry, ELISA
Options selector
Catalog no. Size Conjugation
A02527-2 100 ug/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • 100 ug/vial / Carrier Free, 100 ug/vial / Unconjugated: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.; Form: Lyophilized; Applications: ELISA,WB; Application details: Western blot, 0.25-0.5μg/ml, Rat; ELISA, 0.1-0.5μg/ml,; Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.
    • 100 ug/vial / APC, 100 ug/vial / Biotin, 100 ug/vial / Cy3, 100 ug/vial / FITC, 100 ug/vial / Fluoro488, 100 ug/vial / Fluoro550, 100 ug/vial / Fluoro594, 100 ug/vial / Fluoro647, 100 ug/vial / PE: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.; Form: Liquid; Applications: Flow Cytometry, WB,IHC,ELISA; Application details: Flow Cytometry, 1-3μg/1x106 cells; Western blot, 0.25-0.5μg/ml; Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml; ELISA, 0.1-0.5μg/ml; Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing., At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
    • 100 ug/vial / HRP: Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.; Form: Liquid; Applications: WB,IHC,ELISA; Application details: Western blot, 0.25-0.5μg/ml; Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml; ELISA, 0.1-0.5μg/ml; Storage: At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing.
  • Lead time: varies by selected option; please contact us for current fulfillment timing.
  • Storage: varies by selected option; see option details above.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible; avoid repeated freeze-thaw cycles.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Cd244
Alternative names Natural killer cell receptor 2B4; Cd244; LOC685808
UniProt # E9PT21
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • Rabbit IgG
Reactivity
  • Rat
Applications
  • Western Blot
  • ELISA
Immunogen E.coli-derived rat 2B4/Cd244 recombinant protein (Position: Q20-Q372).
Purification Immunogen affinity purified.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Cellular localization integral component of membrane
Concentration Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Form Lyophilized
Storage Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) A02527-2
Main SKU BHA21002402
Rabbit Polyclonal · Picoband®

Product Overview

This rabbit polyclonal antibody targets CD244 molecule in rat samples and is validated for Western blot and ELISA.

CD244, also known as 2B4, is a SLAM family receptor expressed on NK cells, some CD8+ T cells and myeloid cells. It binds CD48 and can deliver activating or inhibitory signals depending on the adaptor proteins present, so it is studied in NK-cell cytotoxicity and T-cell exhaustion.

Target CD244 molecule (gene Cd244; UniProt E9PT21, rat)
Host / Clonality / Isotype Rabbit / Polyclonal / Rabbit IgG
Reactivity Rat
Purification Immunogen affinity purified
Form Lyophilized
Formulation Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3
Reconstitution 500 µg/mL after adding 0.2 mL distilled water
Storage As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 0.25–0.5 µg/mL (Rat)
ELISA Not specified by the supplier

Immunogen

Recombinant rat 2B4/Cd244 fragment (Gln20–Gln372), expressed in E. coli.

Reactivity Notes

The supplier lists reactivity with rat. UniProt places CD244 molecule in the membrane.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 0.25–0.5 µg/mL (Rat); ELISA (dilution not specified). Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Rat. Other species are not listed by the supplier.
Q.What immunogen was used to raise this antibody?
A.Recombinant rat 2B4/Cd244 fragment (Gln20–Gln372), expressed in E. coli.
Q.How should this antibody be stored?
A.As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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