| Field | Specification |
|---|---|
| Target | |
| Alternative names | Polyunsaturated fatty acid 5-lipoxygenase; Arachidonate 5-lipoxygenase; 5-LO; 5-lipoxygenase; ALOX5; LOG5 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human 5 Lipoxygenase |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AECE-1 is a rabbit monoclonal antibody that recognizes polyunsaturated fatty acid 5-lipoxygenase (gene ALOX5) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 78.0 kDa, and the supplier reports an observed band at about 78 kDa.
5-lipoxygenase (ALOX5) oxygenates arachidonic acid to 5-HPETE and then converts it to leukotriene A4, the first two steps in leukotriene synthesis. Leukotrienes are potent mediators of inflammation and bronchoconstriction, so ALOX5 is studied in asthma, allergy and cardiovascular inflammation.
| Target | Polyunsaturated fatty acid 5-lipoxygenase (gene ALOX5; UniProt P09917, human) |
|---|---|
| Clone | AECE-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 78.0 kDa |
| Observed MW | 78 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): human K562 cells, human RT4 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human 5 Lipoxygenase.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places polyunsaturated fatty acid 5-lipoxygenase in the cytoplasm, nucleus matrix, nuclear membrane and nucleus envelope. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
