| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5-hydroxytryptamine receptor 2A;5-HT-2;5-HT-2A;Serotonin receptor 2A;HTR2A;HTR2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT2A Receptor , different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets 5-hydroxytryptamine receptor 2A (gene HTR2A) in human, mouse and rat samples and is validated for IHC and Western blot. The predicted molecular weight is 52.6 kDa, and the supplier reports an observed band at about 60 kDa.
The serotonin 2A receptor (5-HT2A, HTR2A) is a G protein-coupled receptor that couples to Gq/G11 and activates phospholipase C, raising intracellular Ca2+. It is abundant in the cerebral cortex and is the main target of psychedelic compounds such as LSD and psilocybin, as well as of many atypical antipsychotics, so it is central to neuropharmacology research.
| Target | 5-hydroxytryptamine receptor 2A (gene HTR2A; UniProt P28223, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 52.6 kDa |
| Observed MW | 60 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
Samples with a confirmed band (WB): human U87 cells, rat brain tissue, mouse brain tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human cerebral cortex tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Synthetic peptide from the C-terminal region of human 5HT2A Receptor. It differs from the mouse sequence at three residues and from the rat sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 5-hydroxytryptamine receptor 2A in the cell membrane, cell projection, cytoplasmic vesicle and membrane. Tissue expression noted by UniProt (human): Detected in brain cortex (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Rodriguez Guadalupe et al. (2023) Histone deacetylase inhibitors mitigate antipsychotic risperidone-induced motor side effects in aged mice and in a mouse model of Alzheimer’s disease. Frontiers in Psychiatry. 10.3389/fpsyt.2022.1020831
Ming Liu et al. (2012) Fluoxetine Attenuates Chronic Methamphetamine-induced Pulmonary Arterial Remodelling: Possible Involvement of Serotonin Transporter and Serotonin 1B Receptor. Basic & Clinical Pharmacology & Toxicology. 10.1111/j.1742-7843.2012.00933.x
Tongjun Ma et al. (2023) Regulatory Effect of Electroacupuncture on Hypothalamic Serotonin and its Receptor in Rats with Cerebral Ischemia. Current Neurovascular Research. 10.2174/1567202620666230612110156
