| Field | Specification |
|---|---|
| Target | |
| Alternative names | 5-hydroxytryptamine receptor 2B;5-HT-2B;5-HT2B;Serotonin receptor 2B;HTR2B; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human 5HT2B Receptor, different from the related mouse sequence by six amino acids, and from the related rat sequence by nine amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects 5-hydroxytryptamine receptor 2B (gene HTR2B) in human and rat samples and is validated for Western blot. The predicted molecular weight is 54.3 kDa, and the supplier reports an observed band at about 54 kDa.
The serotonin 2B receptor (5-HT2B, HTR2B) is a Gq/G11-coupled G protein-coupled receptor that activates phospholipase C signaling. It is expressed in the heart, gut and other peripheral tissues as well as in the brain, and it is studied in cardiac development, heart valve biology and the side effects of serotonergic drugs.
| Target | 5-hydroxytryptamine receptor 2B (gene HTR2B; UniProt P41595, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 54.3 kDa |
| Observed MW | 54 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|
Samples with a confirmed band (WB): rat brain tissue, U87 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human 5HT2B Receptor. It differs from the mouse sequence at six residues and from the rat sequence at nine residues.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places 5-hydroxytryptamine receptor 2B in the cell membrane and synapse. Tissue expression noted by UniProt (human): Ubiquitous. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
