Anti-67kDa Laminin Receptor RPSA Rabbit Monoclonal Antibody

SKU:BHA21008736
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-RPSA antibody (Rabbit monoclonal, clone AOAO-18, Rabbit IgG). Validated for Western blot, immunohistochemistry, immunofluorescence, immunoprecipitation and flow cytometry. Reactivity: Human, Mouse, Rat. Recognizes small ribosomal subunit protein uS2.
Target RPSA
clone number AOAO-18
Host Rabbit
Reactivity Human,Mouse,Rat
Isotype Rabbit IgG
Application(s) WB, IHC, ICC, IF, IP, Flow
Options selector
Catalog no. Size Conjugation
M01691 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      Form: Liquid
      Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      Applications: WB,IHC,ICC,IF,IP,Flow Cytometry
      Application details: WB 1:500-2000<br>IHC 1:50-200<br>ICC/IF 1:50-200<br>IP 1:20<br>FC 1:20<br>
      Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in ~2-3 business days; timing may vary by selected option.
  • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at the recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target RPSA
Alternative names 40S ribosomal protein SA ;37 kDa laminin receptor precursor ;37LRP ;37/67 kDa laminin receptor ;LRP/LR ;67 kDa laminin receptor ;67LR ;Colon carcinoma laminin-binding protein;Laminin receptor 1 ;LamR ;Laminin-binding protein precursor p40 ;LBP/p40 ;Multidrug resistance-associated protein MGr1-Ag;NEM/1CHD4;RPSA ;LAMBR, LAMR1;
UniProt # P08865
Host Rabbit
Clone AOAO-18
Clonality
  • Monoclonal
Isotype
  • Rabbit IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Flow Cytometry
Immunogen A synthesized peptide derived from human 67kDa Laminin Receptor
Molecular weight 32.9 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cell membrane. Cytoplasm. Nucleus . 67LR is found at the surface of the plasma membrane, with its C-terminal laminin- binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Colocalizes with PPP1R16B in the cell membrane. .
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M01691
Main SKU BHA21008736
Rabbit Monoclonal

Product Overview

Clone AOAO-18 is a rabbit monoclonal antibody that recognizes small ribosomal subunit protein uS2 (gene RPSA) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 32.9 kDa, and the supplier reports an observed band at about 40 kDa.

RPSA (uS2) is a small-subunit ribosomal protein required for 40S assembly and late processing of 18S rRNA. It is also found at the cell surface, where it is known as the 37/67 kDa laminin receptor and mediates adhesion to the basement membrane; this form has also been studied as a receptor for prions and some viruses.

Target Small ribosomal subunit protein uS2 (gene RPSA; UniProt P08865, human)
Clone AOAO-18
Host / Clonality / Isotype Rabbit / Monoclonal / Rabbit IgG
Reactivity Human, Mouse, Rat
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 32.9 kDa
Observed MW 40 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunohistochemistry 1:50–1:200
Immunocytochemistry / Immunofluorescence 1:50–1:200
Immunoprecipitation 1:20
Flow cytometry 1:20

Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.

Immunogen

Synthetic peptide based on the sequence of human 67kDa Laminin Receptor.

Reactivity Notes

The supplier lists reactivity with human, mouse and rat. UniProt places small ribosomal subunit protein uS2 in the cell membrane, cytoplasm and nucleus.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunohistochemistry 1:50–1:200; Immunocytochemistry / Immunofluorescence 1:50–1:200; Flow cytometry 1:20; Immunoprecipitation 1:20. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human, Mouse, Rat. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 32.9 kDa; observed band at about 40 kDa. A difference from the calculated size is common and can reflect post-translational modification, processing or isoform differences.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human 67kDa Laminin Receptor.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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