| Field | Specification |
|---|---|
| Target | |
| Alternative names | Neutral cholesterol ester hydrolase 1; NCEH; Acetylalkylglycerol acetylhydrolase; 2-acetyl MAGE hydrolase; Arylacetamide deacetylase-like 1; NCEH1; AADACL1; KIAA1363 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human AADACL1/NCEH1 recombinant protein (Position: Q44-D352). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets neutral cholesterol ester hydrolase 1 (gene NCEH1) in human samples and is validated for Western blot, flow cytometry and ELISA. The predicted molecular weight is 45.8 kDa, and the supplier reports an observed band at about 46 kDa.
NCEH1 (neutral cholesterol ester hydrolase 1, also called KIAA1363 or AADACL1) is an ER-associated serine hydrolase. It hydrolyzes 2-acetyl monoalkylglycerol ether in the platelet-activating factor pathway and cholesteryl esters in macrophages, and it can detoxify organophosphorus compounds. It is studied in lipid metabolism and foam cell formation.
| Target | Neutral cholesterol ester hydrolase 1 (gene NCEH1; UniProt Q6PIU2, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 45.8 kDa |
| Observed MW | 46 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human) |
|---|---|
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human A431 cells, human PC-3 cells, human SIHA cells, human A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with a confirmed signal (flow cytometry): HepG2 cells.
Immunogen
Recombinant human AADACL1/NCEH1 fragment (Gln44–Asp352), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human. UniProt places neutral cholesterol ester hydrolase 1 in the cell membrane and microsome. Tissue expression noted by UniProt (human): Expressed in monocyte-derived macrophages. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
