| Field | Specification |
|---|---|
| Target | |
| Alternative names | 4-aminobutyrate aminotransferase, mitochondrial; (S)-3-amino-2-methylpropionate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; GABA-T; L-AIBAT; ABAT; GABAT |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ABAT |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 22A05 is a rabbit monoclonal antibody that recognizes 4-aminobutyrate aminotransferase, mitochondrial (gene ABAT) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 56.4 kDa, and the supplier reports an observed band at about 50 kDa.
4-Aminobutyrate aminotransferase (ABAT, GABA-T) is a mitochondrial enzyme that breaks down the inhibitory neurotransmitter GABA to succinate semialdehyde. By controlling GABA levels in the brain it is an important target in neuroscience, and it is inhibited by the antiepileptic drug vigabatrin.
| Target | 4-aminobutyrate aminotransferase, mitochondrial (gene ABAT; UniProt P80404, human) |
|---|---|
| Clone | 22A05 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 56.4 kDa |
| Observed MW | 50 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:50 |
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ABAT.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places 4-aminobutyrate aminotransferase, mitochondrial in the mitochondrion matrix. Tissue expression noted by UniProt (human): Liver > pancreas > brain > kidney > heart > placenta. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
