| Field | Specification |
|---|---|
| Target | |
| Alternative names | ATP-binding cassette sub-family G member 8;Sterolin-2;ABCG8; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human ABCG8, different from the related mouse and rat sequences by twelve amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets ATP-binding cassette sub-family G member 8 (gene ABCG8) in human, mouse and rat samples and is validated for ICC/IF and Western blot. The predicted molecular weight is 75.7 kDa, and the supplier reports an observed band at about 76 kDa.
ABCG8 pairs with ABCG5 to form a sterol transporter in the intestine and liver that returns cholesterol and plant sterols to the gut lumen and secretes them into bile. The heterodimer is essential for sterol balance, and ABCG8 variants are linked to sitosterolemia and gallstones.
| Target | ATP-binding cassette sub-family G member 8 (gene ABCG8; UniProt Q9H221, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 75.7 kDa |
| Observed MW | 76 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): human HL-60 cells, human K562 cells, human HepG2 cells, rat liver tissue, rat PC-12 cells, mouse liver tissue, mouse HEPA1–6 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): HepG2 cells.
Immunogen
Synthetic peptide from the middle region of human ABCG8. It differs from the mouse and rat sequences at twelve residues.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places ATP-binding cassette sub-family G member 8 in the cell membrane and apical cell membrane. Tissue expression noted by UniProt (human): Predominantly expressed in the liver. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Qing Zhang et al. (2023) Overexpression of LOX-1 in hepatocytes protects vascular smooth muscle cells from phenotype transformation and wire injury induced carotid neoatherosclerosis through ALOX15. Biochimica et Biophysica ACTA-Molecular Basis of Disease. 10.1016/j.bbadis.2023.166805
Guanqi Dai et al. (2023) Mettl3-mediated m6A modification plays a role in lipid metabolism disorders and progressive liver damage in mice by regulating lipid metabolism-related gene expression. Aging-US. 10.18632/aging.204810
