| Field | Specification |
|---|---|
| Target | |
| Alternative names | Acetyl-CoA carboxylase 1; ACC1; Acetyl-Coenzyme A carboxylase alpha; ACC-alpha; ACACA; ACAC; ACCA |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human ACACA. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets acetyl-CoA carboxylase 1 (gene ACACA) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 265.6 kDa, and the supplier reports an observed band at about 266 kDa.
Acetyl-CoA carboxylase 1 (ACACA, ACC1) is a biotin-dependent cytosolic enzyme that converts acetyl-CoA to malonyl-CoA, the first and rate-limiting step of fatty acid synthesis. It is controlled by AMPK phosphorylation and is a target in studies of lipogenesis, metabolic disease and cancer metabolism.
| Target | Acetyl-CoA carboxylase 1 (gene ACACA; UniProt Q13085, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 265.6 kDa |
| Observed MW | 266 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|
Samples with a confirmed band (WB): human HeLa cells, human A549 cells, rat brain tissue, mouse brain tissue, mouse NIH/3T3 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide from the C-terminal region of human ACACA.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places acetyl-CoA carboxylase 1 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wang Shuai et al. (2018) 6-Gingerol Ameliorates Behavioral Changes and Atherosclerotic Lesions in ApoE−/− Mice Exposed to Chronic Mild Stress. Cardiovascular Toxicology. 10.1007/s12012-018-9452-4
