| Field | Specification |
|---|---|
| Target | |
| Alternative names | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2; Centaurin-beta-2; Cnt-b2; ACAP2; CENTB2; KIAA0041 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ACAP2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30A18 is a rabbit monoclonal antibody that recognizes arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 (gene ACAP2) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 88.0 kDa, and the supplier reports an observed band at about 88 kDa.
ACAP2 (centaurin beta-2) is a GTPase-activating protein for ARF6 that contains coiled-coil, ankyrin repeat and PH domains. By switching off ARF6 it helps control endosomal membrane trafficking and recycling, and it is studied in membrane transport and cell signaling.
| Target | Arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 (gene ACAP2; UniProt Q15057, human) |
|---|---|
| Clone | 30A18 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 88.0 kDa |
| Observed MW | 88 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): 293 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ACAP2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places arf-GAP with coiled-coil, ANK repeat and PH domain-containing protein 2 in the cell membrane and endosome membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
