| Field | Specification |
|---|---|
| Target | |
| Alternative names | Acetyl-CoA acetyltransferase, cytosolic; Acetyl-CoA transferase-like protein; Cytosolic acetoacetyl-CoA thiolase; ACAT2; ACTL |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ACAT2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27A26 is a rabbit monoclonal antibody that recognizes acetyl-CoA acetyltransferase, cytosolic (gene ACAT2) in human, mouse and rat samples and is validated for Western blot and IHC. The predicted molecular weight is 41.4 kDa, and the supplier reports an observed band at about 41 kDa.
Cytosolic acetyl-CoA acetyltransferase (ACAT2) joins two acetyl-CoA molecules to form acetoacetyl-CoA, an early step of the mevalonate pathway that leads to cholesterol. It is studied in cholesterol synthesis and lipid metabolism.
| Target | Acetyl-CoA acetyltransferase, cytosolic (gene ACAT2; UniProt Q9BWD1, human) |
|---|---|
| Clone | 27A26 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 41.4 kDa |
| Observed MW | 41 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with a confirmed band (WB): human K562 cells, human HepG2 cells, rat lung tissue, rat kidney tissue, mouse lung tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ACAT2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places acetyl-CoA acetyltransferase, cytosolic in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Mohammed Said Moose Al-Bulish et al. (2022) Docosahexaenoic acid-rich fish oil alleviates hepatic steatosis in association with regulation of gut microbiome in ob/ob mice. Food Research International. 10.1016/j.foodres.2022.111373
