| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiotensin-converting enzyme; ACE; Dipeptidyl carboxypeptidase I; Kininase II; CD antigen CD143; DCP; DCP1 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ACE recombinant protein (Position: R149-C545). Human ACE shares 90.4% and 91.4% amino acid (aa) sequence identity with mouse and rat ACE, respectively. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets angiotensin-converting enzyme (gene ACE) in human, mouse and rat samples and is validated for Western blot, IHC, flow cytometry and ELISA.
Angiotensin-converting enzyme (ACE) is a zinc peptidase that cleaves angiotensin I to the vasoconstrictor angiotensin II and inactivates bradykinin. This makes it a key regulator of blood pressure and fluid balance, and it is the target of ACE inhibitor drugs.
| Target | Angiotensin-converting enzyme (gene ACE; UniProt P12821, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human, Mouse) |
| ELISA | 0.1–0.5 µg/mL |
| Flow cytometry | Not specified by the supplier |
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human ACE fragment (Arg149–Cys545), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 90.4% and 91.4%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places angiotensin-converting enzyme in the cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate...
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
