| Field | Specification |
|---|---|
| Target | |
| Alternative names | Angiotensin-converting enzyme; ACE; Dipeptidyl carboxypeptidase I; Kininase II; CD antigen CD143; DCP; DCP1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ACE1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ADCO-1 is a rabbit monoclonal antibody that recognizes angiotensin-converting enzyme (gene ACE) in human and mouse samples and is validated for Western blot, IHC and flow cytometry. The predicted molecular weight is 149.7 kDa, and the supplier reports an observed band at about 180 kDa.
Angiotensin-converting enzyme (ACE) is a zinc peptidase that cleaves angiotensin I to the vasoconstrictor angiotensin II and inactivates bradykinin. This makes it a key regulator of blood pressure and fluid balance, and it is the target of ACE inhibitor drugs.
| Target | Angiotensin-converting enzyme (gene ACE; UniProt P12821, human) |
|---|---|
| Clone | ADCO-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 149.7 kDa |
| Observed MW | 180 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Flow cytometry | 1:20 |
Samples with a confirmed band (WB): human fetal kidney, rat lung tissue, mouse lung tissue, mouse kidney tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:500, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ACE1.
Reactivity Notes
The supplier lists reactivity with human and mouse. UniProt places angiotensin-converting enzyme in the cell membrane and cytoplasm. Tissue expression noted by UniProt (human): Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate... The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
