| Field | Specification |
|---|---|
| Target | |
| Alternative names | Heterogeneous nuclear ribonucleoprotein K;hnRNP K;Transformation up-regulated nuclear protein;TUNP;HNRNPK;HNRPK; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Acetyl-CoA Carboxylase ACC1 a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system. Catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACC Alpha (ACC1) is the predominant isoform found in liver, adipocytes, and mammary gland, while the 280 kDa ACC Beta (ACC2) is the major isoform in skeletal muscle and heart. |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone EDI-1 is a rabbit monoclonal antibody that recognizes ACACA in human, mouse and rat samples and is validated for Western blot and IHC.
| Target | ACACA (UniProt Q13085/O00763) |
|---|---|
| Clone | EDI-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Observed MW | 265 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
Samples with confirmed staining (IHC): human kidney tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Immunogen
Synthetic peptide based on the sequence of human acetyl-CoA Carboxylase ACC1 a subunit of acetyl-CoA carboxylase (ACC), a multifunctional enzyme system.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xin Li et al. (2021) A flavonoid-rich Smilax china L. extract prevents obesity by upregulating the adiponectin-receptor/AMPK signalling pathway and modulating the gut microbiota in mice. Food & Function. 10.1039/D1FO00282A
Xiangxiang Li et al. (2022) Quinacrine attenuates diet-induced obesity by inhibiting adipogenesis via activation of AMPK signaling. European Journal of Pharmacology. 10.1016/j.ejphar.2022.175432
Ying Zhang et al. (2018) miR-1322 regulates ChREBP expression via binding a 3′-UTR variant (rs1051943). Journal of Cellular and Molecular Medicine. 10.1111/jcmm.13805
Hui Zhang et al. (2021) Down-regulation of ACACA suppresses the malignant progression of Prostate Cancer through inhibiting mitochondrial potential. Journal of Cancer. 10.7150/jca.49560
Ying Zhang et al. (2020) Trimetazidine improves hepatic lipogenesis and steatosis in non‑alcoholic fatty liver disease via AMPK‑ChREBP pathway. Molecular Medicine Reports. 10.3892/mmr.2020.11309
Bingyu Ye et al. (2019) ACC1 is overexpressed in liver cancers and contributes to the proliferation of human hepatoma Hep G2 cells and the rat liver cell line BRL 3A. Molecular Medicine Reports. 10.3892/mmr.2019.9994
