| Field | Specification |
|---|---|
| Target | |
| Alternative names | Cytoplasmic aconitate hydratase;Aconitase;4.2.1.3;Citrate hydro-lyase;Ferritin repressor protein;Iron regulatory protein 1;IRP1;Iron-responsive element-binding protein 1;IRE-BP 1;ACO1;IREB1; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Aconitase 1 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone ABG-1 is a rabbit monoclonal antibody that recognizes cytoplasmic aconitate hydratase (gene ACO1) in human, mouse and rat samples and is validated for Western blot and ICC/IF. The predicted molecular weight is 98.4 kDa, and the supplier reports an observed band at about 98 kDa.
Cytoplasmic aconitase (ACO1), also called iron regulatory protein 1 (IRP1), switches between two roles depending on iron. With an iron-sulfur cluster it acts as an aconitase; without it, it binds iron-responsive elements in mRNAs to control proteins such as ferritin and transferrin receptor.
| Target | Cytoplasmic aconitate hydratase (gene ACO1; UniProt P21399, human) |
|---|---|
| Clone | ABG-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 98.4 kDa |
| Observed MW | 98 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Immunogen
Synthetic peptide based on the sequence of human aconitase 1.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places cytoplasmic aconitate hydratase in the cytoplasm.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
