| Field | Specification |
|---|---|
| Target | |
| Alternative names | Long-chain-fatty-acid--CoA ligase 1;6.2.1.3;Acyl-CoA synthetase 1;ACS1;Long-chain acyl-CoA synthetase 1;LACS 1;Long-chain acyl-CoA synthetase 2;LACS 2;Long-chain fatty acid-CoA ligase 2;Palmitoyl-CoA ligase 1;Palmitoyl-CoA ligase 2;ACSL1;FACL1, FACL2, LACS, LACS1, LACS2; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E. coli-derived human ACSL1 recombinant protein (Position: D604-V698). Human ACSL1 shares 81.1% and 86.3% amino acid (aa) sequence identity with mouse and rat ACSL1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects long-chain-fatty-acid--CoA ligase 1 (gene ACSL1) in human, mouse and rat samples and is validated for flow cytometry, ICC/IF, IHC and Western blot. The predicted molecular weight is 77.9 kDa, and the supplier reports an observed band at about 78 kDa.
Long-chain acyl-CoA synthetase 1 (ACSL1) activates long-chain fatty acids by attaching coenzyme A, a required step before they can be oxidized or built into lipids. It is highly expressed in liver, fat, heart and muscle and is studied in fatty acid oxidation and lipid storage.
| Target | Long-chain-fatty-acid--CoA ligase 1 (gene ACSL1; UniProt P33121, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 77.9 kDa |
| Observed MW | 78 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human, Mouse, Rat) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
Samples with a confirmed band (WB): human HepG2 cells, human U87 cells, rat liver tissue, rat heart tissue, mouse liver tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): mouse kidney tissue, rat kidney tissue, human liver cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Samples with confirmed staining (ICC/IF): U2OS cells.
Samples with a confirmed signal (flow cytometry): A431 cells.
Immunogen
Recombinant human ACSL1 fragment (Asp604–Val698), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 81.1% and 86.3%, respectively.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places long-chain-fatty-acid--CoA ligase 1 in the mitochondrion outer membrane, peroxisome membrane, microsome membrane and endoplasmic reticulum membrane. Tissue expression noted by UniProt (human): Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xin Zhou et al. (2023) Transcriptomics and Molecular Docking Reveal the Potential Mechanism of Lycorine Against Pancreatic Cancer. Phytomedicine. 10.1016/j.phymed.2023.155128
