| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Long-chain-fatty-acid--CoA ligase 1;6.2.1.3;Acyl-CoA synthetase 1;ACS1;Long-chain acyl-CoA synthetase 1;LACS 1;Long-chain acyl-CoA synthetase 2;LACS 2;Long-chain fatty acid-CoA ligase 2;Palmitoyl-CoA ligase 1;Palmitoyl-CoA ligase 2;ACSL1;FACL1, FACL2, LACS, LACS1, LACS2; |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Host | |
| Immunogen | E. coli-derived human ACSL1 recombinant protein (Position: D604-V698). Human ACSL1 shares 81.1% and 86.3% amino acid (aa) sequence identity with mouse and rat ACSL1, respectively. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Reconstitution | |
| Target | |
| UniProt # |
Overview
This antibody is intended for detection of ACSL1 (Long-chain-fatty-acid--CoA ligase 1) in biological samples using common immunoassay formats. It is typically selected based on target identity, species reactivity, clonality/clone information, and detection modality.
Vendor notes: Boster Bio Anti-ACSL1 Antibody Picoband® catalog # PB10025. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Key elements and design rationale
- Antibody format: Rabbit Polyclonal Rabbit IgG
- Immunogen / epitope context: E. coli-derived human ACSL1 recombinant protein (Position: D604-V698). Human ACSL1 shares 81.1% and 86.3% amino acid (aa) sequence identity with mouse and rat ACSL1, respectively. (reported region: D604-V698).
- Molecular weight context: reported MW: 78 kDa; calculated MW: 77943 MW
- Reactivity: Human,Mouse,Rat
- Applications: Flow Cytometry, IF, IHC, ICC, WB
As a polyclonal antibody, the reagent recognizes multiple epitopes on the target, which can improve detection robustness but may increase sensitivity to sample-dependent epitope changes.
Biological background
Long-chain-fatty-acid--CoA ligase 1; Long-chain-fatty-acid--CoA ligase 1. Long-chain-fatty-acid—CoA ligase 1 is an enzyme that in humans is encoded by the ACSL1 gene. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. Several transcript variants encoding different isoforms have been found for this gene. This specific protein is most commonly found in mitochondria and peroxisomes. Functional note: Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Reported localization: Mitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein . Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein . Expression/tissue context: Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas. .
Research relevance and current trends
- Cancer: Researchers commonly examine how ACSL1 (Long-chain-fatty-acid--CoA ligase 1) relates to this theme using model systems and orthogonal readouts.
- Cancer Metabolism: Researchers commonly examine how ACSL1 (Long-chain-fatty-acid--CoA ligase 1) relates to this theme using model systems and orthogonal readouts.
- Cardiovascular: Researchers commonly examine how ACSL1 (Long-chain-fatty-acid--CoA ligase 1) relates to this theme using model systems and orthogonal readouts.
Common research applications
- Western blotting: compare relative ACSL1 (Long-chain-fatty-acid--CoA ligase 1) levels across conditions; band patterns may reflect isoforms and processing.
- IHC/IHC-F: assess spatial distribution of ACSL1 (Long-chain-fatty-acid--CoA ligase 1) across tissue regions and cell types using matched controls.
- IF/ICC: evaluate subcellular localization and co-localization patterns; signal can depend on fixation/permeabilization and epitope accessibility.
- Flow cytometry: quantify target-positive populations and shifts in expression; gating strategy and background staining controls are essential.
Notes for experimental interpretation
- Specificity notes: No cross reactivity with other proteins.
- Cross-reactivity: No cross-reactivity with other proteins
- Isoforms and PTMs: Apparent size and signal patterns can differ across splice isoforms, proteolytic processing, and post-translational modifications.
- Controls: Include an isotype control (as relevant), no-primary control for imaging, and orthogonal validation such as KD/KO samples when available.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
