| Field | Specification |
|---|---|
| Target | |
| Alternative names | Long-chain-fatty-acid--CoA ligase 5; Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 5; LACS 5; ACSL5; ACS5; FACL5 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ACSL5 recombinant protein (Position: Y108-D683). Human ACSL5 shares |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets long-chain-fatty-acid--CoA ligase 5 (gene ACSL5) in human, mouse and rat samples and is validated for Western blot, ICC/IF, flow cytometry, immunoprecipitation and ELISA.
Long-chain acyl-CoA synthetase 5 (ACSL5) activates long-chain fatty acids to acyl-CoAs and is highly expressed in the small intestine. It is thought to help channel dietary fatty acids toward triglyceride synthesis, and it is studied in intestinal lipid metabolism and epithelial turnover.
| Target | Long-chain-fatty-acid--CoA ligase 5 (gene ACSL5; UniProt Q9ULC5, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Purification | Immunogen affinity purified |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Reconstitution | 500 µg/mL after adding 0.2 mL distilled water |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
| Immunoprecipitation | 0.5–2 µg/mL (Human) |
| Flow cytometry (fixed cells) | 1–3 µg/1×106 cells (Human) |
| ELISA | 0.1–0.5 µg/mL |
Immunogen
Recombinant human ACSL5 fragment (Tyr108–Asp683), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places long-chain-fatty-acid--CoA ligase 5 in the mitochondrion, endoplasmic reticulum, mitochondrion outer membrane and endoplasmic reticulum membrane.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
