| Field | Specification |
|---|---|
| Target | |
| Gene symbol | |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This monoclonal antibody (clone 5B7), raised in Mouse, recognizes ACTB. It is validated for Immunofluorescence, Western Blot, Immunohistochemistry.
Validated Applications
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry |
|---|---|
| Optimal working dilutions should be determined experimentally by the investigator Suggested starting dilutions are as follows | IHC 1:200 IF 1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
See specifications table.
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | 5B7 |
| Isotype | IgG1 |
| Conjugate | AbFluor™ 488 |
| Reactivity | Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Insect |
| Specificity | Beta-actin Monoclonal Antibody (5B7) AbFluor™ 488 Conjugated specially designed for your Immunofluorescence analysis. |
| Purification | The antibody was purified using affinity-chromatography using specific immunogen. |
| Concentration | 1 mg/mL |
| Formulation | Liquid in PBS pH7.4, containing 0.02% Sodium Azide and 50% Glycerol. |
| UniProt # | P60709 |
| Gene ID | 60 |
| Storage | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Insect. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for ACTB research from St John's Laboratory Ltd, or contact us for alternative formats.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
