| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-actinin-2;Alpha-actinin skeletal muscle isoform 2;F-actin cross-linking protein;ACTN2; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ACTN2 |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AOCB-1 is a rabbit monoclonal antibody that recognizes alpha-actinin-2 (gene ACTN2) in human, mouse and rat samples and is validated for Western blot, IHC and immunoprecipitation. The predicted molecular weight is 103.9 kDa, and the supplier reports an observed band at about 104 kDa.
Alpha-actinin-2 (ACTN2) is the main alpha-actinin of cardiac and skeletal muscle. It cross-links actin filaments at the Z-disc and anchors the sarcomere, and variants in ACTN2 are associated with cardiomyopathies.
| Target | Alpha-actinin-2 (gene ACTN2; UniProt P35609, human) |
|---|---|
| Clone | AOCB-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 103.9 kDa |
| Observed MW | 104 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
Samples with a confirmed band (WB): HepG2 cells, rat skeletal muscle tissue, rat heart tissue, mouse skeletal muscle tissue, mouse heart tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ACTN2.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places alpha-actinin-2 in the cytoplasm. Tissue expression noted by UniProt (human): Expressed in both skeletal and cardiac muscle. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Xu Yi et al. (2024) Fructose aggravates copper-deficiency-induced cardiac remodeling by inhibiting SERCA2a. Journal of Pharmacy and Pharmacology. 10.1093/jpp/rgae002
