Anti-ACTR1B Rabbit Monoclonal Antibody

SKU:BHA21010549
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Suppliers
Boster Bio
Boster Bio
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Overview
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Anti-ACTR1B antibody (Rabbit monoclonal, clone 30A59, IgG). Validated for Western blot, immunohistochemistry, immunofluorescence, flow cytometry and immunoprecipitation. Reactivity: Human. Recognizes beta-centractin. Observed band in Western blot: about 42 kDa.
Target ACTR1B
clone number 30A59
Host Rabbit
Reactivity Human
Isotype IgG
Application(s) WB, IHC, IF, ICC, FC, IP
Options selector
Catalog no. Size Conjugation
M12745 100 uL/vial
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options:
    • Size: 100 uL/vial; Conjugation: Unconjugated
      • Storage: Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.
      • Form: Liquid
      • Applications: WB,IHC,ICC,IF,IP,Flow Cytometry
      • Application details: WB 1:500-2000
        IHC 1:50-200
        ICC/IF 1:50-200
        IP 1:50
        FC 1:50
      • Contents: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
  • Lead time: typically ships in 2-3 business days; timing may vary by selected option.
  • Storage: Refer to the product datasheet for storage and handling.
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: follow the product datasheet storage instructions.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target ACTR1B
Alternative names Beta-centractin; Actin-related protein 1B; ARP1B; ACTR1B; CTRN2
Gene ID 10120
UniProt # P42025
Host Rabbit
Clone 30A59
Clonality
  • Monoclonal
Isotype
  • IgG
Reactivity
  • Human
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • Flow Cytometry
  • Immunoprecipitation
Immunogen A synthesized peptide derived from human ACTR1B
Molecular weight 42.3 kDa
Purification Affinity-chromatography
Reconstitution Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Cellular localization Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
Concentration 0.5mg/ml
Form Liquid
Storage Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Catalog no. (Mfr.) M12745
Main SKU BHA21010549
Rabbit Monoclonal

Product Overview

Clone 30A59 is a rabbit monoclonal antibody that recognizes beta-centractin (gene ACTR1B) in human samples and is validated for Western blot, IHC, ICC/IF, flow cytometry and immunoprecipitation. The predicted molecular weight is 42.3 kDa, and the supplier reports an observed band at about 42 kDa.

Beta-centractin (ACTR1B, Arp1B) is an actin-related protein of the dynactin complex. Dynactin works with the motor dynein to move vesicles and organelles along microtubules and is associated with the centrosome, so ACTR1B is studied in intracellular transport.

Target Beta-centractin (gene ACTR1B; UniProt P42025, human)
Clone 30A59
Host / Clonality / Isotype Rabbit / Monoclonal / IgG
Reactivity Human
Form Liquid
Formulation PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers
Calculated MW 42.3 kDa
Observed MW 42 kDa
Storage Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Validated Applications

Western blot 1:500–1:2000
Immunohistochemistry 1:50–1:200
Immunocytochemistry / Immunofluorescence 1:50–1:200
Immunoprecipitation 1:50
Flow cytometry 1:50

Samples with a confirmed band (WB): 293 cells.

Recommended loading (WB): 20–40 µg of total protein per lane.

Immunogen

Synthetic peptide based on the sequence of human ACTR1B.

Reactivity Notes

The supplier lists reactivity with human. UniProt places beta-centractin in the cytoplasm. The samples tested by the supplier (listed above) are a practical starting point for positive controls.

Safety

Handling: the formulation contains sodium azide as a preservative. Wear gloves, avoid contact with skin and eyes, and dispose of waste according to local regulations. For research use only; not for diagnostic or therapeutic procedures.
Q.Which applications is this antibody validated for, and at what dilution?
A.Validated applications with suggested starting dilutions: Western blot 1:500–1:2000; Immunohistochemistry 1:50–1:200; Immunocytochemistry / Immunofluorescence 1:50–1:200; Flow cytometry 1:50; Immunoprecipitation 1:50. Optimal dilutions should be determined by each laboratory.
Q.Which species does this antibody react with?
A.Validated reactivity: Human. Other species are not listed by the supplier.
Q.What band size should be expected in Western blot?
A.Reported by the supplier: calculated molecular weight 42.3 kDa; observed band at about 42 kDa.
Q.What immunogen was used to raise this antibody?
A.Synthetic peptide based on the sequence of human ACTR1B.
Q.How should this antibody be stored?
A.Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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