| Field | Specification |
|---|---|
| Target | |
| Alternative names | Activin receptor type-2A; Activin receptor type IIA; ACTR-IIA; ACTRIIA; ACVR2A; ACVR2 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ACVR2A |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25A00 is a rabbit monoclonal antibody that recognizes activin receptor type-2A (gene ACVR2A) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 57.8 kDa, and the supplier reports an observed band at about 85 kDa.
Activin receptor type-2A (ACVR2A) is a type II serine/threonine kinase receptor for activins, myostatin and other TGF-β family ligands. After ligand binding it phosphorylates type I receptors, which then activate SMAD proteins, and it is studied in reproduction, muscle growth and bone.
| Target | Activin receptor type-2A (gene ACVR2A; UniProt P27037, human) |
|---|---|
| Clone | 25A00 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 57.8 kDa |
| Observed MW | 85 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): human HeLa cells, human RT4 cells, human SH-SY5Y cells, human 293T cells, rat brain tissue, rat C6 cells, mouse brain tissue, mouse Neuro-2a cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 10% SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 1:500 overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Synthetic peptide based on the sequence of human ACVR2A.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places activin receptor type-2A in the cell membrane. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
