| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Alternative Names | Activin A Receptor Like Type 1, ALK1, Serine/Threonine-Protein Kinase Receptor R3, SKR3, TGF-B Superfamily Receptor Type I, TSR-I |
| Clonality | |
| Conjugate | |
| Host | |
| Isotype | |
| Product Type | |
| Reactivity | |
| Shipping | |
| Storage | |
| Target |
Overview
Anti-ACVRL1 (extracellular) Antibody is an antibody targeting Activin A Receptor Like Type 1, ALK1, Serine/Threonine-Protein Kinase Receptor R3, SKR3, TGF-B Superfamily Receptor Type I, TSR-I Polyclonal raised in Rabbit (Unconjugated). This antibody is commonly used in IFC, LCI, WB to detect, localize, or compare expression of the target across samples.
Key elements and design rationale
- Target: Activin A Receptor Like Type 1, ALK1, Serine/Threonine-Protein Kinase Receptor R3, SKR3, TGF-B Superfamily Receptor Type I, TSR-I (also reported as Activin A Receptor Like Type 1, ALK1, Serine/Threonine-Protein Kinase Receptor R3, SKR3, TGF-B Superfamily Receptor Type I, TSR-I).
- Immunogen/epitope region: Extracellular, N-terminus..
- Homology note: Mouse, rat - 14/15 identical. The antibody will not work with human samples. (informative for cross-species interpretation).
- Species reactivity (as provided): Rat, Mouse.
- Lot quality control (as provided): Western blot analysis..
- Peptide confirmation: Confirmed by amino acid analysis and mass spectrometry.
- Blocking peptide: Available for antigen preadsorption control where appropriate.
- Conjugate/format: Unconjugated (may affect detection channel and background).
These attributes help researchers interpret whether signal reflects the intended target in a given assay and sample context.
Biological background
ACVRL1, commonly known as Activin Receptor-Like Kinase 1 (ALK1), is a type I receptor in the Transforming Growth Factor-beta (TGF-β) superfamily. This receptor belongs to a family of serine/threonine kinase receptors, which form heteromeric complexes with type II receptors to transduce signals via SMAD transcription factors. ALK1 specifically interacts with Bone Morphogenetic Proteins (BMPs), particularly BMP9 and BMP10, to activate SMAD1/5/8 phosphorylation, regulating endothelial cell proliferation, maturation and migration1-5.
Research relevance and current trends
- Comparing target expression across perturbations, genotypes, or treatment conditions.
- Interpreting localization shifts alongside pathway or phenotypic readouts.
- Using orthogonal controls (KO/KD, peptide competition, isotype concepts) to support conclusions.
Common research applications
- Western blot (WB): compare target abundance/size across lysates and conditions; consider isoforms/PTMs.
- Immunofluorescence/ICC: assess subcellular localization and co-localization with markers in cells or sections.
- Flow cytometry (direct/indirect): quantify target-positive populations and shifts in expression across subsets.
- Live cell imaging (LCI): support extracellular-epitope detection on non-permeabilized cells when appropriate.
Interpretation typically benefits from comparing matched sample sets (e.g., treated vs control, WT vs KO/KD) and using orthogonal readouts where feasible.
Notes for experimental interpretation
- Isoforms and post-translational modifications can shift apparent molecular weight or epitope accessibility across samples.
- Cross-species signal may depend on epitope conservation; consult the provided homology note when selecting models.
- Permeabilization, fixation, and antigen retrieval can change accessibility of intracellular vs extracellular epitopes.
- Conceptual control: antigen preadsorption (blocking peptide) can help assess signal dependence on the immunogen region.
- Provided control suggestions: Negative control: BLP-BR041.
- Application notes: see product-specific dilution/usage notes and control concepts provided in the dataset.
Application abbreviations: CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot. Species abbreviations: H- Human, M- Mouse, R- Rat.
Recommended controls: Blocking peptide: BLP-BR041; Negative control: BLP-BR041.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
