| Field | Specification |
|---|---|
| Target | |
| Alternative names | A disintegrin and metalloproteinase with thrombospondin motifs 5; ADAM-TS 5; ADAM-TS5; ADAMTS-5; A disintegrin and metalloproteinase with thrombospondin motifs 11; ADAM-TS 11; ADAMTS-11; ADMP-2; Aggrecanase-2; ADAMTS5; ADAMTS11; ADMP2 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ADAMTS5 recombinant protein (Position: D747-K780). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody targets A disintegrin and metalloproteinase with thrombospondin motifs 5 (gene ADAMTS5) in human, mouse and rat samples and is validated for Western blot, IHC and ELISA. The predicted molecular weight is 101.7 kDa, and the supplier reports an observed band at about 75 kDa.
ADAMTS5 (aggrecanase-2) is a secreted metalloprotease that degrades aggrecan, versican and related proteoglycans of the extracellular matrix. In mouse studies it is a key enzyme of cartilage aggrecan loss, making it an important target in osteoarthritis research.
| Target | A disintegrin and metalloproteinase with thrombospondin motifs 5 (gene ADAMTS5; UniProt Q9UNA0, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 101.7 kDa |
| Observed MW | 75 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.25–0.5 µg/mL (Human, Mouse, Rat) |
|---|---|
| IHC (paraffin sections) | 2–5 µg/mL (Human) |
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): human HeLa cells, human HepG2 cells, human U2OS cells, human SW620 cells, human THP-1 cells, human Raji cells, human SGC-7901 cells, rat liver tissue, rat testis tissue, mouse liver tissue, mouse testis tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (IHC): human placenta tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in EDTA buffer (pH 8.0).
Immunogen
Recombinant human ADAMTS5 fragment (Asp747–Lys780), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. Tissue expression noted by UniProt (human): Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Yuxiang Hu et al. (2025) Denosumab attenuates knee osteoarthritis progression by inhibiting synovial inflammation via the RANK/TRAF6/FSTL1 signalling. Nature Communications. 10.1038/s41467-025-66202-z
Kai Sun et al. (2024) TRPM2-mediated feed-forward loop promotes chondrocyte damage in osteoarthritis via calcium-cGAS-STING-NF-κB pathway. Journal of Advanced Research. 10.1016/j.jare.2024.11.007
Shenghao Cai et al. (2024) Self-healing hydrogels loaded with Spatholobi Caulis alleviate disc degeneration by promoting autophagy in nucelus pulposus. Materials Today Bio. 10.1016/j.mtbio.2024.101323
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Bowei Ni et al. (2021) MCC950, the NLRP3 Inhibitor, Protects against Cartilage Degradation in a Mouse Model of Osteoarthritis. Oxidative Medicine and Cellular Longevity. 10.1155/2021/4139048
Taiyang Liao et al. (2024) Total glucosides of white paeony capsule alleviate articular cartilage degeneration and aberrant subchondral bone remodeling in knee osteoarthritis. Phytotherapy Research. 10.1002/ptr.8210
Rui Lu et al. (2024) TBK1 pharmacological inhibition mitigates osteoarthritis through attenuating inflammation and cellular senescence in chondrocytes. Journal of Orthopaedic Translation. 10.1016/j.jot.2024.06.001
Lu Rui et al. (2021) Physalin A Inhibits MAPK and NF-κB Signal Transduction Through Integrin αVβ3 and Exerts Chondroprotective Effect. Frontiers in Pharmacology. 10.3389/fphar.2021.761922
Pei Wenbin et al. (2021) Selective STAT3 Inhibitor Alantolactone Ameliorates Osteoarthritis via Regulating Chondrocyte Autophagy and Cartilage Homeostasis. Frontiers in Pharmacology. 10.3389/fphar.2021.730312
Rui Lu et al. (2023) Mulberroside A alleviates osteoarthritis via restoring impaired autophagy and suppressing MAPK/NF-κB/PI3K-AKT-mTOR signaling pathways. iScience. 10.1016/j.isci.2023.105936
