| Field | Specification |
|---|---|
| Target | |
| Alternative names | Double-stranded RNA-specific adenosine deaminase;DRADA;3.5.4.37;136 kDa double-stranded RNA-binding protein;p136;Interferon-inducible protein 4;IFI-4;K88DSRBP;ADAR;ADAR1, DSRAD, G1P1, IFI4; |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived human ADAR1 recombinant protein (Position: S128-Q346). Human ADAR1 shares 90.2% and 50.7% amino acid (aa) sequence identity with mouse and rat ADAR1, respectively. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects double-stranded RNA-specific adenosine deaminase (gene ADAR) in human samples and is validated for ICC/IF, IHC and Western blot. The predicted molecular weight is 136.1 kDa; the supplier reports observed bands at 110 kDa, 150 kDa.
ADAR1 (ADAR) is an RNA-editing enzyme that converts adenosine to inosine in double-stranded RNA. By editing the cell's own RNAs it prevents them from being sensed as viral, damping type I interferon responses, and its loss causes Aicardi-Goutières syndrome.
| Target | Double-stranded RNA-specific adenosine deaminase (gene ADAR; UniProt P55265, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 136.1 kDa |
| Observed MW | 110 kDa, 150 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human) |
|---|---|
| IHC (paraffin sections) | 0.5–1 µg/mL (Human) |
| Immunocytochemistry / Immunofluorescence | 5 µg/mL (Human) |
Samples with a confirmed band (WB): HeLa cells, A549 cells, MCF-7 cells, HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 50 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:10000, ECL detection.
Samples with confirmed staining (IHC): human lung cancer tissue.
Recommended antigen retrieval: heat-mediated, in TE buffer (pH 9.0); citrate buffer (pH 6.0) can be used instead.
Conditions in the example images (IHC): heat-mediated antigen retrieval in citrate buffer (pH 6).
Immunogen
Recombinant human ADAR1 fragment (Ser128–Gln346), expressed in E. coli. Sequence identity with the mouse and rat orthologs: 90.2% and 50.7%, respectively.
Reactivity Notes
The supplier lists reactivity with human. Tissue expression noted by UniProt (human): Ubiquitously expressed, highest levels were found in brain and lung. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
