| Field | Specification |
|---|---|
| Target | |
| Alternative names | Adiponectin; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; Adipoq; Acdc; Apm1 |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human Adiponectin |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 30A64 is a rabbit monoclonal antibody that recognizes adiponectin (gene Adipoq) in mouse samples and is validated for Western blot, IHC and ICC/IF. The predicted molecular weight is 26.8 kDa, and the supplier reports an observed band at about 28 kDa.
Adiponectin is a hormone secreted by adipocytes and one of the most abundant adipokines in plasma. Acting through the receptors ADIPOR1 and ADIPOR2, it activates AMPK and increases insulin sensitivity and fatty acid oxidation, and low circulating levels are associated with obesity and type 2 diabetes.
| Target | Adiponectin (gene Adipoq; UniProt Q60994, mouse) |
|---|---|
| Clone | 30A64 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Mouse |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 26.8 kDa |
| Observed MW | 28 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
Samples with a confirmed band (WB): mouse kidney cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human adiponectin.
Reactivity Notes
The supplier lists reactivity with mouse. UniProt describes adiponectin as a secreted protein. Tissue expression noted by UniProt (mouse): Synthesized exclusively by adipocytes and secreted into plasma. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
