| Field | Specification |
|---|---|
| Target | |
| Alternative names | Adiponectin receptor protein 1;Progestin and adipoQ receptor family member I;ADIPOR1;PAQR1 , TESBP1A;CGI-45; |
| Gene ID | |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human ADIPOR1, different from the related mouse sequence by two amino acids. |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody recognizes adiponectin receptor protein 1 (gene ADIPOR1) in human and rat samples and is validated for ICC/IF and Western blot. The predicted molecular weight is 42.6 kDa, and the supplier reports an observed band at about 43 kDa.
Adiponectin receptor 1 (ADIPOR1) is a seven-transmembrane receptor for the fat-derived hormone adiponectin. Its signaling activates AMPK, increasing fatty acid oxidation and glucose uptake, and it is highly expressed in skeletal muscle, making it a focus of metabolic and diabetes research.
| Target | Adiponectin receptor protein 1 (gene ADIPOR1; UniProt Q96A54, human) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Human, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 0.9 mg NaCl; 0.2 mg Na2HPO4; 0.05 mg NaN3; plus stabilizers |
| Calculated MW | 42.6 kDa |
| Observed MW | 43 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.5 µg/mL (Human, Rat) |
|---|---|
| Immunocytochemistry / Immunofluorescence | 2 µg/mL (Human) |
Samples with a confirmed band (WB): rat thymus tissue, rat testis tissue, MCF-7 cells, A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.5 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Samples with confirmed staining (ICC/IF): U2OS cells.
Immunogen
Synthetic peptide from the N-terminal region of human ADIPOR1. It differs from the mouse sequence at two residues.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places adiponectin receptor protein 1 in the cell membrane. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
