| Field | Specification |
|---|---|
| Target | |
| Alternative names | Adenosine kinase; AK; Adenosine 5'-phosphotransferase; N6,N6-dimethyladenosine kinase; N6-isopentenyladenosine kinase; N6-methyladenosine kinase; ADK |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human ADK |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 25A83 is a rabbit monoclonal antibody that recognizes adenosine kinase (gene ADK) in human and rat samples and is validated for Western blot. The predicted molecular weight is 40.5 kDa, and the supplier reports an observed band at about 46 kDa.
Adenosine kinase (ADK) phosphorylates adenosine to AMP and is the main enzyme that clears adenosine inside cells. By controlling adenosine levels it influences neuromodulation and methylation reactions, and it is studied in epilepsy, liver metabolism and inflammation.
| Target | Adenosine kinase (gene ADK; UniProt P55263, human) |
|---|---|
| Clone | 25A83 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 40.5 kDa |
| Observed MW | 46 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): HepG2 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human ADK.
Reactivity Notes
The supplier lists reactivity with human and rat. UniProt places adenosine kinase in the cytoplasm and SUBCELLULAR LOCATION:. Tissue expression noted by UniProt (human): Widely expressed. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
