| Field | Specification |
|---|---|
| Target | |
| Alternative names | Alpha-fetoprotein;Alpha-1-fetoprotein;Alpha-fetoglobulin;AFP;HPAFP; |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AFP |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone AAOO-1 is a rabbit monoclonal antibody that recognizes alpha-fetoprotein (gene AFP) in human samples and is validated for Western blot, IHC, ICC/IF, immunoprecipitation and flow cytometry. The predicted molecular weight is 68.7 kDa, and the supplier reports an observed band at about 68 kDa.
Alpha-fetoprotein (AFP) is the major serum protein of the fetus, produced mainly by the fetal liver and yolk sac and related in structure to albumin. It binds fatty acids, metal ions and bilirubin, and its levels are widely measured in studies of fetal development and liver tumors such as hepatocellular carcinoma.
| Target | Alpha-fetoprotein (gene AFP; UniProt P02771, human) |
|---|---|
| Clone | AAOO-1 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / Rabbit IgG |
| Reactivity | Human |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 68.7 kDa |
| Observed MW | 68 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Immunoprecipitation | 1:20 |
| Flow cytometry | 1:20 |
Immunogen
Synthetic peptide based on the sequence of human AFP.
Reactivity Notes
The supplier lists reactivity with human. UniProt describes alpha-fetoprotein as a secreted protein. Tissue expression noted by UniProt (human): Plasma.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Wu Rongrong et al. (2011) Derivation of multipotent nestin+/CD271−/STRO-1− mesenchymal-like precursors from human embryonic stem cells in chemically defined conditions. Human Cell. 10.1007/s13577-011-0022-3
