| Field | Specification |
|---|---|
| Target | |
| Alternative names | Advanced glycation end product-specific receptor; Receptor for advanced glycation end products; Ager; Rage |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | E.coli-derived mouse Ager recombinant protein (Position: Q24-E320). |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Lyophilized |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
This rabbit polyclonal antibody detects advanced glycation end product-specific receptor (gene Ager) in mouse and rat samples and is validated for Western blot and ELISA. The predicted molecular weight is 42.7 kDa; the supplier reports observed bands at 43 kDa, 58 kDa.
RAGE (AGER, receptor for advanced glycation end products) is a cell surface pattern recognition receptor of the immunoglobulin superfamily. It binds diverse stress-related ligands, including advanced glycation end products, S100 proteins, HMGB1 and amyloid-β oligomers, and triggers inflammatory signaling, so it is studied in diabetes complications, vascular disease, neurodegeneration and chronic inflammation.
| Target | Advanced glycation end product-specific receptor (gene Ager; UniProt Q62151, mouse) |
|---|---|
| Host / Clonality / Isotype | Rabbit / Polyclonal / Rabbit IgG |
| Reactivity | Mouse, Rat |
| Form | Lyophilized |
| Formulation | Per vial: 4 mg trehalose; 0.9 mg NaCl; 0.2 mg Na2HPO4 |
| Calculated MW | 42.7 kDa |
| Observed MW | 43 kDa, 58 kDa |
| Storage | As supplied: −20 °C for up to 12 months from receipt. After reconstitution: 4 °C for up to 1 month, or aliquot and keep at −20 °C for up to 6 months. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 0.1–0.25 µg/mL (Mouse, Rat) |
|---|---|
| ELISA | 0.1–0.5 µg/mL |
Samples with a confirmed band (WB): rat lung tissue, mouse lung tissue.
Recommended loading (WB): 20–40 µg of total protein per lane.
Conditions in the example images (WB): 5–20% gradient SDS-PAGE under reducing conditions, 30 µg lysate per lane, transfer to nitrocellulose membrane, blocking in 5% non-fat milk, primary antibody at 0.25 µg/mL overnight at 4 °C, HRP-conjugated secondary antibody at 1:5000, ECL detection.
Immunogen
Recombinant mouse Ager fragment (Gln24–Glu320), expressed in E. coli.
Reactivity Notes
The supplier lists reactivity with mouse and rat. UniProt places advanced glycation end product-specific receptor in the cell membrane, cell projection, early endosome and nucleus. Tissue expression noted by UniProt (mouse): Isoform 1: Expressed at higher levels in the coronary arterioles in type 2 diabetic mice (at protein level). The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
