| Field | Specification |
|---|---|
| Target | |
| Alternative names | Glycogen debranching enzyme; Glycogen debrancher; AGL; GDE |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from human AGL |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 27A59 is a rabbit monoclonal antibody that recognizes glycogen debranching enzyme (gene AGL) in human, mouse and rat samples and is validated for Western blot, IHC, ICC/IF and flow cytometry. The predicted molecular weight is 174.8 kDa, and the supplier reports an observed band at about 175 kDa.
Glycogen debranching enzyme (AGL) carries two activities, a glucanotransferase and an amylo-1,6-glucosidase, that together remove branch points during glycogen breakdown. Loss of AGL function causes glycogen storage disease type III, making it a focus of research on glycogen metabolism in liver and muscle.
| Target | Glycogen debranching enzyme (gene AGL; UniProt P35573, human) |
|---|---|
| Clone | 27A59 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 174.8 kDa |
| Observed MW | 175 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|---|
| Immunohistochemistry | 1:50–1:200 |
| Immunocytochemistry / Immunofluorescence | 1:50–1:200 |
| Flow cytometry | 1:50 |
Samples with a confirmed band (WB): K562 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of human AGL.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places glycogen debranching enzyme in the cytoplasm. Tissue expression noted by UniProt (human): Liver, kidney and lymphoblastoid cells express predominantly isoform 1. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
