| Field | Specification |
|---|---|
| Target | |
| Alternative names | Adenosylhomocysteinase; AdoHcyase; S-adenosyl-L-homocysteine hydrolase; AHCY; SAHH |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | A synthesized peptide derived from AHCY |
| Molecular weight | |
| Purification | |
| Reconstitution | |
| Cellular localization | |
| Concentration | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
Clone 26A42 is a rabbit monoclonal antibody that recognizes adenosylhomocysteinase (gene AHCY) in human, mouse and rat samples and is validated for Western blot. The predicted molecular weight is 47.7 kDa, and the supplier reports an observed band at about 48 kDa.
Adenosylhomocysteinase (AHCY) hydrolyzes S-adenosylhomocysteine, the by-product of SAM-dependent methylation reactions, into adenosine and homocysteine. Because S-adenosylhomocysteine inhibits methyltransferases, AHCY activity supports cellular methylation capacity and is studied in one-carbon metabolism and epigenetics.
| Target | Adenosylhomocysteinase (gene AHCY; UniProt P23526, human) |
|---|---|
| Clone | 26A42 |
| Host / Clonality / Isotype | Rabbit / Monoclonal / IgG |
| Reactivity | Human, Mouse, Rat |
| Form | Liquid |
| Formulation | PBS (pH 7.4); 150 mM NaCl; 0.02% sodium azide; 50% glycerol; plus stabilizers |
| Calculated MW | 47.7 kDa |
| Observed MW | 48 kDa |
| Storage | Long term: −20 °C for up to 12 months. Frequent use: 4 °C for up to 1 month. Avoid repeated freeze–thaw cycles. |
Validated Applications
| Western blot | 1:500–1:2000 |
|---|
Samples with a confirmed band (WB): A549 cells.
Recommended loading (WB): 20–40 µg of total protein per lane.
Immunogen
Synthetic peptide based on the sequence of AHCY.
Reactivity Notes
The supplier lists reactivity with human, mouse and rat. UniProt places adenosylhomocysteinase in the cytoplasm, melanosome, nucleus and endoplasmic reticulum. The samples tested by the supplier (listed above) are a practical starting point for positive controls.
Safety
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
Junyao Zhou et al. (2022) Metabolomics and integrated network pharmacology analysis reveal that ginkgolides act as potential active anticancer components by regulating one-carbon metabolism. Journal of Ethnopharmacology. 10.1016/j.jep.2022.115609
